| 小麦幼穗愈伤组织诱导与植株再生的研究 |
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| 引用本文： | 赵元增,茹振钢,李友勇. 小麦幼穗愈伤组织诱导与植株再生的研究[J]. 河南农业科学, 2004, 0(2): 3-6 |
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| 作者姓名： | 赵元增 茹振钢 李友勇 |
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| 作者单位： | 河南职业技术师范学院农学系,河南,新乡,453003 |
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| 摘 要： | 以 3个小麦品种的幼穗为外植体 ,选用 9种培养基 ,研究了影响小麦幼穗愈伤组织诱导和分化的因素。结果表明 :小麦幼穗愈伤组织诱导和再分化都存在基因型效应 ;外源激素的种类和浓度也是影响小麦幼穗愈伤组织诱导和再分化的重要因素。在愈伤组织诱导中 ,较低的2 ,4-D浓度 ( 0 .5～ 1 .0mg/L)也能达到较好的诱导效果 ,但 2 ,4-D最适宜的使用浓度为2 .0mg/L ,细胞分裂素KT或 6-BA不利于愈伤组织的诱导。
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| 关 键 词： | 小麦 幼穗 愈伤组织诱导 分化 |
| 文章编号： | 1004-3268(2004)02-0003-04 |
| 修稿时间： | 2003-10-16 |
Studies on Callus Induction and Plant Regeneration from Wheat Immature Spike |
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| ZHAO Yuan zeng,RU Zhen gang,LI You yong. Studies on Callus Induction and Plant Regeneration from Wheat Immature Spike[J]. Journal of Henan Agricultural Sciences, 2004, 0(2): 3-6 |
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| Authors: | ZHAO Yuan zeng RU Zhen gang LI You yong |
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| Abstract: | In lab test the explants of wheat immature spike of three wheat varieties were cultured on nine mediums,the results showed that callus induction and regeneration was affected mainly by wheat genotype , and the categories and concentration of exogenous hormone were important effect factors. The concentration of 2,4-D ranged from 0.5 mg/L to 1.0 mg/L was suited for callus induction ,but the best concentration was 2.0 mg/L,6-BA or KT was not suitable for callus induction. |
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| Keywords: | Wheat Immature spike Callus induction Plant regeneration |
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