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不同样品分散温度对双波长比色法测定木薯直、支链淀粉比率及总淀粉含量的影响
引用本文:黄惠芳,罗燕春,候学文,符海天,彭靖茹,黎萍,黄强.不同样品分散温度对双波长比色法测定木薯直、支链淀粉比率及总淀粉含量的影响[J].中国农学通报,2012,28(3):272-276.
作者姓名:黄惠芳  罗燕春  候学文  符海天  彭靖茹  黎萍  黄强
作者单位:1. 广西壮族自治区亚热带作物研究所,南宁530001;广西壮族自治区木薯研究所,南宁530001
2. 华南农业大学,广州,510642
基金项目:广西科技厅主席基金资助课题“高淀粉产量木薯分子育种技术研究”(主席基金09203-03)
摘    要:为了研究木薯直、支链淀粉比率及总淀粉含量的测定方法,通过采用双波长比色法,研究不同样品分散温度对木薯淀粉直、支链淀粉比率及总淀粉含量的影响。结果表明:在试验温度70~100℃范围内,85℃时直链淀粉百分比率最低,相对应地,85℃时支链淀粉的百分比率最高。样品分散温度对鲜薯中总淀粉含量测定值有影响,其测定值在80℃时最高。由此可知,不同样品分散温度对木薯淀粉直、支链淀粉比率及总淀粉含量测定值有影响。

关 键 词:木薯  木薯  干旱胁迫  土壤相对含水量  光合作用  光响应  
收稿时间:8/4/2011 12:00:00 AM
修稿时间:2011/11/4 0:00:00

The Affection that Different Temperature of Sample Dispersion to Ratio of Amylose and Amylopectin, Total Starch Content with Double Wavelength Spectrophotometric on Cassava
Huang Huifang , Luo Yanchun , Hou Xuewen , Fu Haitian , Su Wenpan , Peng Jingru , Li Ping , Huang Qiang.The Affection that Different Temperature of Sample Dispersion to Ratio of Amylose and Amylopectin, Total Starch Content with Double Wavelength Spectrophotometric on Cassava[J].Chinese Agricultural Science Bulletin,2012,28(3):272-276.
Authors:Huang Huifang  Luo Yanchun  Hou Xuewen  Fu Haitian  Su Wenpan  Peng Jingru  Li Ping  Huang Qiang
Institution:1 Guangxi Zhuang Autonomous Region Research Institute for Subtropical Crops , Nanning 530001; 2 Guangxi Zhuang Autonomous Region Research Institute for Cassava , Nanning 530001; 3 South China Agricultural University , Guangzhou 510642)
Abstract:In order to determine the amylose and amylopectin, total starch content of cassava roots by double wavelength spectrophotometric method. The effect of different sample dispersion temperatures on cassava amylose and amylopectin, total starch content by double wavelength spectrophotometric method were studied. The results were as follows: the percentage of amylose was the lowest when the sample dispersion temperature was 85℃, however the percentage of amylopectin was the highest, among the sample dispersion temperature used. When the sample dispersion temperature was 80℃, the determined total starch content was the highest. These results demonstrated that the effects of sample dispersion temperatures on the determination of amylose, amylopectin, total starch content were different.
Keywords:cassava  starch content  double wavelengths  temperature of the sample dispersion
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