利用SSR标记研究南斯拉夫玉米自交系遗传变异及杂种优势群预测

 利用113对SSR多态性引物对9个南斯拉夫玉米自交系和国内18个不同类群玉米自交系进行遗传多样性分析,进而研究GD,GH,SCA及杂种优势与F₁产量的相关性,探讨预测杂种优势的可能性。结果表明:（1）利用SSR标记将27个供试自交系分为6类,我国自交系的分类结果与其系谱来源和以往研究结果基本一致,PA,PB和四平头类群自交系分别各归为一类;南斯拉夫自交系ZPL01,ZPL06,ZPL09,ZPL10和ZPL02归为第Ⅰ类, ZPL03,ZPL05,ZPL04与Mo17和丹232归为第Ⅱ类群, ZPL07与综3和综31归为第Ⅲ类;（2）SCA与对照优势及其与F₁穗粒重呈极显著正相关,而GD,GH与F₁穗粒重和对照优势的相关仅长葛点达到显著水平,利用SCA或对照优势可以较好地预测F₁的产量表现,而GD和GH的预测效果较差。

Study on the Genetic Diversity of Introduced Yugoslavia Maize Inbred Lines and Prediction of Heterosis Using SSR Markers

Exotic germplasms have been playing an important role in corn breeding and production in our country. But to make good use of them, their genetic characteristics and germplasm relationship with Chinese inbred lines representing different heterotic groups should be studied. In this study, the genetic diversity of 27 elite maize inbred lines, 9 introduced Yugoslavia inbreds and 7 Chinese inbreds representing different heterotic groups, were evaluated by 113 polymorphism SSR markers. And the correlations among GD, GH, SCA, over-check heterosis and F_1 grain yield, and their abilities and results in heterosis prediction were also studied. The results showed that all the 27 inbred lines were classified into 6 clusters, which was in agreement with pedigree analysis. All PA, PB and Tangsipingtou lines were assigned into their groups accordingly; Yugoslavia lines ZPL01, ZPL02, ZPL06, ZPL09 and ZPL10 were placed into cluster I, ZPL03, ZPL04, ZPL05 into cluster II with Mo17 and Dan232, ZPL07 into cluster III with Zong3 and Zong31. There were significantly positive correlations among SCA, heterosis and F_1 ear-kernel weight, but those GD, GH and F_1 ear-kernel weight or over-check heterosis were significant only at Changge. So SCA and over-check heterosis could be used to predict F_1 yield, but the ability of GD and GH in heterosis predication was limited and easily affected by environment. The correlation between GD and GH was significantly positive, but they had low correlation with SCA.