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| 安徽砀山酥梨自然保护区梨种质资源AFLP分析 | |
| 引用本文： | 朱立武,王艳芳,贾兵,张水明,黄永丰,杨振江. 安徽砀山酥梨自然保护区梨种质资源AFLP分析[J]. 果树学报, 2009, 26(2) |
| 作者姓名： | 朱立武 王艳芳 贾兵 张水明 黄永丰 杨振江 |
| 作者单位： | 朱立武,王艳芳,贾兵,张水明,ZHU Li-wu,WANG Yan-fang,JIA Bing,ZHANG Shui-ming(安徽农业大学果树学重点实验室,安徽合肥,230036);黄永丰,杨振江,HUANG Yong-feng,YANG Zhen-Jiang(安徽省砀山酥梨自然保护区管理委员会,安徽砀山,235300) |
| 基金项目： | 国家公益性行业(农业)科研专项基金，安徽省教育厅优秀人才计划 |
| 摘 要： | 为探讨安徽砀山酥梨自然保护区梨种质资源遗传多样性和鉴定砀山酥梨营养系变异,以该保护区内27个梨资源代表类型为试材,利用AFLP分子标记技术开展研究。运用从36对选择性扩增引物中筛选的3对引物组合进行扩增,每对引物可扩增出50条以上的清晰条带,扩增片段长度为30～600bp,9个引物组合共扩增出清晰可靠的DNA谱带540条。其中498条为多态性条带,平均多态性比率为92.2%。聚类分析表明,当遗传距离阈值为39.2～40.1时,全部供试生物型可分为6类:第1类为砀山酥梨及其变异类型和原产砀山的地方品种、杂交种;第2类包括雪花梨、鸭梨、紫酥、慈梨、早美酥、丰水、新高,白梨和砂梨聚在了一类;第3类包括库尔勒香梨、红香酥;南果梨、红巴梨、杜梨各为一类。与砀山酥梨相比,砀山酥梨的8个营养系变异类型均扩增出了特异带,特异条带出现的数量为5～15条不等。说明安徽砀山酥梨自然保护区梨种质资源遗传多样性丰富;砀山酥梨8个营养系变异类型均在DNA水平发生了不同程度的突变。 |
| 关 键 词： | 梨 扩增片段长度多态性 聚类分析 突变 |
Study on the pear germplasm resources in Dangshan area,Anhui province by AFLP technology |
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| ZHU Li -wu,WANG Yan -fang,JIA Bing,ZHANG Shui -ming,HUANG Yong -feng,YANG Zhen -Jiang. Study on the pear germplasm resources in Dangshan area,Anhui province by AFLP technology[J]. Journal of Fruit Science, 2009, 26(2) | |
| Authors: | ZHU Li -wu WANG Yan -fang JIA Bing ZHANG Shui -ming HUANG Yong -feng YANG Zhen -Jiang |
| Affiliation: | 1Key Laboratory of Pomology;Anhui Agricultural University;Hefei;Anhui 230036 China;2Dangshansu Pear Germplasm Resources Protec-tion District Administration Committee;Dangshan;Anhui 235300 China |
| Abstract: | Total of 27 biotypes of pear,cultivated in the Protection District of Dangshansu Pear,Dangshan County,Anhui Province were analyzed by the established technical system of amplified fragment length polymorphism(AFLP)for studying the genetic diversity and mutant's identification in genus Pyrus.Three pairs of selective primer screened out from 36 pairs of selective ones were used in the amplification and more than 50 bands were amplified by each pair of selective primer.The size of amplified fragment was 30 to ... |
| Keywords: | Pear AFLP Cluster analysis Mutant |
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