| 仿刺参补体类似物活性的测定 |
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| 引用本文： | 张峰,宫晶,王海峰,赵啸,李宣延.仿刺参补体类似物活性的测定[J].大连水产学院学报,2007,22(4):246-248. |
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| 作者姓名： | 张峰 宫晶 王海峰 赵啸 李宣延 |
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| 作者单位： | 1. 大连水产学院,生命科学与技术学院,辽宁,大连,116023
2. 辽宁出入境检验检疫局,辽宁,大连,116001 |
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| 摘 要： | 用3种方法对仿刺参Apostichopus japonicus体腔液、体腔液上清液及体腔细胞中的总补体溶血活性和补体类似物AjC3、AjC4活性进行测定.结果表明:在仿刺参体腔液、体腔液上清液和体腔细胞中能够检测到总补体溶血活性和补体类似物AjC3、AjC4,表明仿刺参体内存在有脊椎动物的补体类似物,且活性与正常人补体活性相似.仿刺参体内总补体溶血活性和补体类似物AjC3、AjC4的活性依次顺序均为体腔液＞体腔细胞＞体腔液上清液.
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| 关 键 词： | 仿刺参 体腔液 体腔细胞 补体类似物AjC3、AjC4 溶血活性 |
| 文章编号： | 1000-9957（2007）04-0246-03 |
| 修稿时间： | 2006-01-30 |
The detection of complement analogues AjC3, and AjC4 in the sea cumcumber Apostichopus japonicus |
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| ZHANG Feng,GONG Jing,WANG Hai-feng,ZHAO Xiao,LI Xuan-yan.The detection of complement analogues AjC3, and AjC4 in the sea cumcumber Apostichopus japonicus[J].Journal of Dalian Fisheries University,2007,22(4):246-248. |
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| Authors: | ZHANG Feng GONG Jing WANG Hai-feng ZHAO Xiao LI Xuan-yan |
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| Institution: | 1. School of Life Seienee and Technology, Dalian Fisheries Univ. , Dalian 116023, China; 2. Entri- Exit Inspection and Quarantine Bureau, Dalian 116001 ,China |
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| Abstract: | The hemolytic activity and complement analogues AjC3, and AjC4 in coelomic fluid, the supernatant of coelomic fluid and coelomocytes were tested in sea cucumber Apostichopus japonicus by three methods. The results showed that there were complement analogues, AjC3, and AjC4 in the coelomic fluid, the supernatant of coelomic fluid and coelomocytes in the sea cucumber. The results indicated that an activity of complement analogues was present in the sea cucumber,and the activity of complement analogues was same as the activity of mammalian one. There was the maximal complement analogues AjC3, AjC4 in the coelomic fluid, followed by in the coelomocytes and the minimum in the supematant of the coelomic fluid. |
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| Keywords: | Apostichopus japonicus coelomic fluid coelomocytes complement analogues AjC3 AjC4 hemolytic activity |
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