尾分析法检测梅花鹿产茸量性状关联的RAPD标记

作者以18只均配有产茸记录的梅花鹿为试验对象,将其按产茸量分为高、低两组,提取每组单个个体血样的DNA后,对该DNA模板进行PCR 扩增。发现混合DNA所扩增出的条带包含了所有单个个体DNA扩增的条带,说明混合DNA可替代单个DNA进行群体遗传结构分析;另外,比较各性状表型值高、低组的混合DNA扩增片段后发现,在高、低产茸量的鹿群里分别发现了1、3个RAPD标记与性状关联。结果表明尾分析法可用于数量性状位点的标记检测。

Detection of the Linkage between RAPD Markers and Antler Production in Spotted Deer by Tail Analysis Method

The 18 spotted deers were studied,and they had been reported about antler production.According to antler production,high and low group were divided in the population.DNA isolated from each blood was used to amplify with PCR technique.All bands amplified with individual DNA was found in mixed DNA,which showed mixed DNA could be used to analyze the genetic structure of population in place of individual DNA.And compared with mixed DNA amplified bands of high and low group of each trait,there were 1 or 3 RAPD marker-traits association in spotted deers population.These results indicated that tail analysis method could be used to detect QTL.