| 马立克氏病鸡MDV血清基因的克隆与鉴定 |
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| 引用本文： | 高烨,王晓菊,郑梅竹.马立克氏病鸡MDV血清基因的克隆与鉴定[J].安徽农业科学,2012,40(8):4590-4591. |
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| 作者姓名： | 高烨 王晓菊 郑梅竹 |
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| 作者单位： | 长春师范学院化学学院,吉林长春,130032;长春师范学院化学学院,吉林长春,130032;长春师范学院化学学院,吉林长春,130032 |
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| 基金项目： | 吉林省教育厅项目，长春师范学院自然科学基金 |
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| 摘 要： | 目的]克隆马立克氏病鸡MDV血清基因,并对其进行鉴定。方法]从潜伏期感染马立克氏病鸡淋巴组织中提取基因组DNA,采用梯度PCR扩增马立克氏病毒的L-meq基因。将其插入pMD18-T克隆载体并进行测序,并用DNAman软件进行序列分析。结果]获得的DNA片段的序列与已发表的L-meq基因序列相一致,表明该试验成功获得了目的基因。结论]该研究可为鸡马立克氏病的预防与治疗提供新思路和新方法。
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| 关 键 词： | 马立克氏病 L-meq基因 扩增 克隆 |
Cloning and Identification of L-meq Gene of Marek' s Disease Virus |
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| Institution: | GAO Ye et al(College of Chemistry,Changchun Normal University,Changchun,Jilin 130032) |
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| Abstract: | Objective] To clone and identify Marek’s disease virus(MDV) serum gene.Method] MDV genomic DNA was extracted from lymphoid tissues of the diseased chickens with latent MDV infection.The MDV L-meq gene was amplified by gradient PCR,inserted into pMD18-T vector and sequenced.The sequence was analyzed using DNAman software.Result] The obtained sequence had 100% similarity with the published sequence of L-meq gene,showing successful amplification of target gene.Conclusion] The paper provides new ideas and new methods for prevention and treatment of Marek’s disease in chickens. |
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| Keywords: | Marek’s disease L-meq gene Amplification Cloning |
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