A rapid and simple method of detection of <Emphasis Type="Italic">Blepharisma japonicum</Emphasis> using PCR and immobilisation on FTA paper期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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A rapid and simple method of detection of <Emphasis Type="Italic">Blepharisma japonicum</Emphasis> using PCR and immobilisation on FTA paper

Authors:	Email author" target="_blank">Geoff?Hide Email author Jacqueline?M?Hughes Robert?McNuff

Institution:	(1) Centre for Parasite Biology, Molecular Epidemiology and Ecology, Biosciences Research Institute, School of Environment and Life Sciences, University of Salford, Salford, UK, M5 4WT;(2) SCIENTO, 61 Bury Old Road, Whitefield, Manchester, UK, M45 6TB

Abstract:	Background The rapid expansion in the availability of genome and DNA sequence information has opened up new possibilities for the development of methods for detecting free-living protozoa in environmental samples. The protozoan Blepharisma japonicum was used to investigate a rapid and simple detection system based on polymerase chain reaction amplification (PCR) from organisms immobilised on FTA paper.

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