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| 寒胁迫诱导灰木相思无性系差异蛋白的表达 | |
| 引用本文： | 何文锦,陈由强,郭晋隆,林思祖,陈如凯.寒胁迫诱导灰木相思无性系差异蛋白的表达[J].福建林学院学报,2007,27(4):355-359. |
| 作者姓名： | 何文锦 陈由强 郭晋隆 林思祖 陈如凯 |
| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350007;福建农林大学林学院,福建,福州,350002 2. 福建师范大学生命科学学院,福建,福州,350007;发育与神经生物学福建省高等学校重点实验室,福建,福州,350108 3. 农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350002 4. 福建农林大学林学院,福建,福州,350002 5. 福建师范大学生命科学学院,福建,福州,350007;农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350002 |
| 基金项目： | 福建省自然科学基金，福建省林业厅科研项目，福建省教育厅资助项目 |
| 摘 要： | 本实验提取寒胁迫前后的灰木相思组培苗的水溶性蛋白进行双向电泳和质谱鉴定,分析在寒胁迫条件下蛋白质组的变化。利用ImageMaster 5.0软件分析比较,经基质辅助激光解吸电离飞行时间质谱测定差异蛋白点的肽质量指纹图谱,通过Mascot软件查询Swiss-Prot等数据库,鉴定得到4个显著差异蛋白点,R7、R15、R20表现为上调表达,R17表现为下调表达。结果表明:在寒胁迫条件下灰木相思组培苗存在明显的蛋白质表达差异。 |
| 关 键 词： | 寒胁迫 灰木相思 蛋白质组学 双向凝胶电泳 MALDI-TOF质谱 |
| 文章编号： | 1001-389X（2007）04-0355-05 |
| 收稿时间： | 2007-05-12 |
| 修稿时间： | 2007-08-20 |
Differential protein expression induced by cold stress the clones of Acacia implexa |
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| HE Wen-jin,CHEN You-qiang,GUO Jin-long,LIN Si-zu,CHEN Ru-kai.Differential protein expression induced by cold stress the clones of Acacia implexa[J].Journal of Fujian College of Forestry,2007,27(4):355-359. | |
| Authors: | HE Wen-jin CHEN You-qiang GUO Jin-long LIN Si-zu CHEN Ru-kai |
| Institution: | 1. College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350007, China; 2. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 3. Key Laboratory of Sugarcane Genetics and Breeding, Ministry of Agriculture, Fuzhou, Fujian 350002, China; 4. State Key Laboratory of Developmental Biology, Fuzhou, Fujian 350108, China |
| Abstract: | There are two different treatment modes in plantlet of Acacia implexa: cold stresss culture and normal temperature culture.Water-soluble proteins were isolated from cold stress culture and normal temperature culture of plantet of A.implexa respectively,then profiled by two-dimensional polyacrylamide gel electrophoresis and software of ImageMaster 5.0,identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).Their Peptide Mass Finger Prints(PMF) were obtained.Further Swiss-Prot database search identified four matched proteins.Among them,three proteins were up-regulated while one protein was down-regulated.The results showed that there were definite differences of protein expresion differences between the cold stress culture and normal temperature culture of plantet of A.implexa. |
| Keywords: | cold stress Acacia implexa proteomics 2-D gel electrophoresis MALDI-TOF-MS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |