水稻OsSsr1基因的生物信息学分析、亚细胞定位及其表达模式

为了探明盐敏感相关基因[OsSsr1（Oryza sativa L.salt-sensitive related gene 1,GenBank登录号为NM₀01065574）]的亚细胞定位及在水稻不同组织和各种逆境胁迫下的表达模式,通过生物信息学手段、洋葱表皮亚细胞定位、RiceXPro数据库和Real-time PCR对其进行研究。序列分析表明OsSsr1的开放阅读框为2 004 bp,编码一个由667个氨基酸组成并含有5个跨膜区的蛋白质,该蛋白质N端含有一个信号肽;其启动子序列包含TGACG-motif、ABRE、TCA-element和W box等多个与逆境相关的顺式作用元件。GFP融合表达显示,OsSSR1蛋白质定位于细胞膜。RiceXPro数据库中数据分析表明,OsSsr1基因在水稻不同发育期的不同组织中均有表达,在叶中的表达量最高,胚中的表达量最低。Real-time PCR结果表明,OsSsr1表达水平受干旱、高温、高盐、低温、机械伤害、稻瘟病菌、MeJA和ABA的诱导上调。这些结果显示,OsSsr1基因可能在水稻胁迫反应中发挥重要作用。

Bioinformatic analysis, subcellular localization and expression pattern of OsSsr1 gene in rice

To analyze the subcellular localization and expression pattern of rice salt-sensitive gene [OsSsr1 gene（Oryza sativa L.salt-sensitive related gene 1,GenBank accession: NM₀01065574）] in different tissues and various environmental stimulus,bioinformatics methods,subcellular localization,RiceXPro database and Real-time PCR were used to study this gene.The sequence analysis showed that OsSsr1 consisted of an ORF with a length of 2 004 bp,encoded a 667 amino acids protein containing five transmembrane regions,and also contained a signal peptide in the N-terminal domain.The promoter sequence contained several stress-related cis-acting elements,such as TGACG-motif,ABRE,TCA-element,and W box.The expression of OsSSR1-GFP fusion protein suggested that OsSSR1 protein mostly appeared in cell membrane.The analysis of expression data in RiceXPro database revealed that OsSsr1 was expressed in different tissues at each development stage of rice,the highest expression in leaf,the lowest in embryo.The real-time PCR analysis indicated that OsSsr1 was up-regulated by kinds of environmental stimulus,including drought,high temperature,high salt,low temperature,wound,Magnaporthe grisea,MeJA and ABA.These findings indicated that OsSsr1 might play an important role in rice resistance to kinds of stresses.