| 板栗疫病菌低毒力遗传转化株LC的构建 |
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| 引用本文： | 蓝秀万,蓝瑛,林海燕,陈保善.板栗疫病菌低毒力遗传转化株LC的构建[J].广西农业生物科学,2007,26(2):106-109,174. |
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| 作者姓名： | 蓝秀万 蓝瑛 林海燕 陈保善 |
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| 作者单位： | 1. 广西大学,生命科学与技术学院
2. 广西大学,生命科学与技术学院;广西大学,广西亚热带生物资源保护利用重点实验室;广西大学,微生物及植物遗传工程教育部重点实验室,广西,南宁,530005 |
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| 基金项目： | 国家自然科学基金
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国家自然科学基金
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教育部高校骨干教师资助计划
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广西自然科学基金 |
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| 摘 要： | 低毒病毒—板栗疫病菌组合是研究病毒—宿主相互作用的模式系统之一。通过构建含板栗疫病菌低毒病毒CHV1-EP713的全长cDNA和苯菌灵抗性基因的表达质粒pXB3F2,并用于转化野生型无毒株EP155,获得了以苯菌灵抗性为筛选标记、具有dsRNA病毒再生能力的低毒力遗传转化株LC,为深入研究病毒—真核宿主相互作用的分子机制提供了新的研究材料。
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| 关 键 词： | 板栗疫病菌 低毒病毒 低毒力遗传转化株LC CHV1-EP713 EP155 |
| 文章编号： | 1008-3464（2007）02-0106-04 |
| 收稿时间： | 2006-05-22 |
| 修稿时间： | 2006-05-222006-06-05 |
Construction of engineered hypovirulent Cryphonectria parasitica strain LC |
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| LAN Xiu-wan,LAN Ying,LIN Hai-yan,CHEN Baoshan.Construction of engineered hypovirulent Cryphonectria parasitica strain LC[J].Journal of Guangxi Agricultural and Biological Science,2007,26(2):106-109,174. |
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| Authors: | LAN Xiu-wan LAN Ying LIN Hai-yan CHEN Baoshan |
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| Institution: | 1 College of Life Science and Technology; 2 Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization; 3 The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Guangxi University, Nanning 530005, China |
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| Abstract: | Hypovirus/chestnut blight fungus(Cryphonectria parasitica) is an excellent experimental system to study the virus-host interaction. pXBn3F2,a plasmid with benomyl resistance gene and the full-length cDNA of Cryphonectria parasitica hypovirus CHV1-EP713,was constructed and used to transform hypovirus-free C. parasitica strain EP155. A resultant transformant designated LC that exhibited benomyl resistance and harbored the resurrected hypovirus CHV1-713 dsRNA was obtained. The availability of the engineered strain LC provides a valuable material for studying the molecular mechanisms of the interaction between hypovirus and its eukaryotic host fungus. |
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| Keywords: | chestnut blight fungus hypovirus engineered hypovirulent strain LC CHV1-EP713 EP155 |
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