| 圆叶椒草芽苗诱导及生长初步研究 |
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| 引用本文： | 王巍,张明生,史梦娜,曹然,许邦锐,丁绍欢,申起飞.圆叶椒草芽苗诱导及生长初步研究[J].贵州大学学报(农业与生物科学版),2012,31(3):207-211. |
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| 作者姓名： | 王巍 张明生 史梦娜 曹然 许邦锐 丁绍欢 申起飞 |
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| 作者单位： | 贵州大学生命科学学院,贵州省药用植物繁育与种植重点实验室,贵州贵阳550025 |
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| 基金项目： | 贵州大学研究生创新项目，贵州省教育厅自然科学研究项目，贵州省中药材现代产业技术体系建设项目 |
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| 摘 要： | 用叶片和茎段为外植体诱导获得的圆叶椒草芽苗,进行其增殖及生长初步研究。结果表明:适于圆叶椒草芽苗增殖和生长的培养条件为:MS+2mg/L 6-BA+0.5mg/L IBA+3%蔗糖+5g/L琼脂+(20±1)℃;适于芽苗生根的培养基为:White+2 mg/L IBA+3%蔗糖+5 g/L琼脂;1/10 Hoagland营养液适于试管苗的液体培养。
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| 关 键 词： | 圆叶椒草 芽苗 诱导 生长 |
A Preliminary Study on Shoots Induction and Growth of Peperomia obtusifolia |
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| WANG Wei
,
ZHANG Ming-sheng
,
SHI Meng-na
,
CAO Ran
,
XU Bang-rui
,
DING Shao-huan
,
SHEN Qi-fei.A Preliminary Study on Shoots Induction and Growth of Peperomia obtusifolia[J].Journal of Mountain Agriculture & Biology,2012,31(3):207-211. |
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| Authors: | WANG Wei ZHANG Ming-sheng SHI Meng-na CAO Ran XU Bang-rui DING Shao-huan SHEN Qi-fei |
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| Institution: | (College of Life Sciences, Guizhou Key Lab of Propagation and Cultivation in Medicinal Plants, Guizhou Uni- versity, Guiyang Guizhou 550025, China) |
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| Abstract: | The shoots induced from leaves and stems of Peperomia obtusifolia were studied preliminarily for the multiplication in the present work. The results showed that the culture conditions suitable for multiplication and growth of the shoots were MS ＋ 2 mg/L 6 - BA ＋ 0.5 mg/L IBA ＋ 3% sucrose ＋ 5 g/L agar. White ＋ 2 mg/L IBA ＋3% sucrose ＋5 g/L agar gave the best effect for root induction. In addition, 1/10 Hoagland nu- trient solution was suitable for the liquid culture of Peperomia obtusifolia plantlets. |
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| Keywords: | Peperomia obtusifolia （L ） A Dietr shoot induction growth |
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