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| 欧洲鳗血清免疫球蛋白纯化及部分特性分析 | |
| 引用本文： | 林天龙.欧洲鳗血清免疫球蛋白纯化及部分特性分析[J].水产学报,2001,25(1):52-57. |
| 作者姓名： | 林天龙 |
| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所, 2. 福建省农业科学院地热农业利用研究所, 3. 华中农业大学水产学院, |
| 基金项目： | 福建省科委重点项目、回国留学人员A类资助项目(97-2-139) |
| 摘 要： | 采用饱和硫酸铵分步盐析法结合柱层析提取，纯化欧洲鳗血清免疫球蛋白（Ｉg) ,实验证实欧洲鳗Ｉg主要分布在硫酸铵饱和度３０％－５０％的区间内，Ｓephacry1-S200进一步提纯的Ｉg主要存在于第一个蛋白峰，而Ｓepharose-4B柱层析提纯的Ｉg则存在于第二个蛋白峰，ＤＥＡE-52脑子交换柱层析可进一步纯化Ｓepharose-4B柱层析的产物，ＥＬＩＳＡ和Western-blot分别证实上述提取物具有抗体的免疫学活性，ＳＤＳ－ＰＡＥＧ分析纯化的Ｉg，显示了洲鳗Ｉg重链约为６８kD，轻链约为２６kD. |
| 关 键 词： | 欧洲鳗 免疫球蛋白 纯化 盐析法 免疫学活性 血清 |
| 文章编号： | 1000-0615(2001)01-0052-06 |
| 收稿时间： | 2014/4/16 0:00:00 |
| 修稿时间： | 2000年3月14日 |
Purification and partial characterization of serum immunoglobulin in Anguilla anguilla |
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| LIN Tian long.Purification and partial characterization of serum immunoglobulin in Anguilla anguilla[J].Journal of Fisheries of China,2001,25(1):52-57. | |
| Authors: | LIN Tian long |
| Institution: | LIN Tian long 1,CHEN Qiang 2,YU Fu song 1,YANG Jin xian 1,GONG Hui 1,LIN Neng feng 1,XU Bin fu 1,YI Guang hui 3 |
| Abstract: | The purification of serum immunoglobulin in European eel(Anguilla anguilla) was carried out by using ammonium sulfate precipitation followed by column chromatography. The results showed that most of the eel serum Ig was precipitated at 30% to 50% ammonium sulfated saturation. Further purification by Sephacryl-S200 showed that the Ig presented in the first protein peak , and by Sepharose 4B the eel Ig existed at the second protein peak; the purified production from Sepharose 4B were further separated by DEAE 52 column into two fractions, and the Ig was found in the first fraction. The antibody activity of the productions stated above was proved by ELISA and Western blot. SDS PAGE showed that the heavy chain and light chain of the eel serum Ig were 68kD and 26kD respectively. |
| Keywords: | Anguilla anguilla immunoglobulin purification |
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