小麦新品种川农12号中外源染色质的分子细胞遗传学检测

为深入了解利用染色体工程新方法选育的小麦新品种川农12号的遗传基础，对其进行Giemsa C-带分析，结果显示川农12号有两条染色体的短臂具有黑麦IRS的端带特征，其长臂与小麦染色体1B的长臂（IBL）带型一致，表明这对染色体可能是IRS／IBL易位染色体；再利用黑麦总基因组DNA作探针，小麦基因组总DNA作封阻对川农12号根尖细胞中期分裂相染色体进行荧光原位杂交，结果证实川农12号确为含IRS／IBL易位染色体的新品种。同时对易位系的选育、IRS／IBL易位的多样性进行了讨论。

Molecular Cytogenetics Identification of Alien Chromatin in Chuannong 12

Giemsa C-banding displayed that a new wheat cultivar, Chuannong 12 that was cultured by chromosome engineering and was planted widely recently in the West Southern of China,had two chromosomes 1RS/1BL.Moreover,Genome in Situ Hybridization(GISH) indicated that two visible signals on the arms of chromosome 1RS/1BL of Chuannong 12.Moreover,Chuannong 12 resisted highly to the powdery mildew disease and stripe rust disease.Therefore,we were sure that Chuannong 12 was 1RS/1BL translocation line.The way of breeding translocation lines and the diversity of 1RS/1BL were discussed.