野生榆叶梅遗传多样性的AFLP分析

本研究利用AFLP分子标记技术,对野生榆叶梅6个居群进行分析.利用已筛选出的8对引物组合进行扩增,共产生256条谱带,其中206条谱带具有遗传多态性,占总谱带的80.47％;平均每对引物组合扩增产生129条多态条带,平均多态率为50.26％.6个居群观察等位基因数Na平均值为1.502 6,总Na值为1.804 7；有...

Genetic Diversity of Wild Populations of Prunus triloba Revealed by AFLP

In this research,AFLP was employed to analyze genetic diversity of 6 wild populations of Prunus triloba.Eight pair primers previous identified were used to amplify specific DNA bands in the six populations.As a result,256 DNA bands were generated and 206 of them were polymorphic bands,which was 80.47% of the total bands.Each primer pairs generated 129 polymorphic bands on average and the mean polymorphism rate was 50.26%.Among the six populations,the total observed number of alleles(Na) was 1.804 7 and the mean was 1.502 6;the effective number of alleles(Ne) was 1.245 8 and the average was 1.216 5;the Nei’s gene diversity index(H) was 0.156 7 and the average H was 0.135 5;the total Shannon’s index(I) was 0.255 4 and the average was 0.213 4.The results of genetic differentiation indicates that 86.47% of the genetic variation should occure among populations while 13.53% should be within a population.The result of AFLP analysis reveals that dendrogram of 6 wild Prunus triloba populations is as similar as the clustering by geographic distance.