Analysis of microtubule and microfilament distribution in Pinus sylvestris roots following infection by Heterobasidion species |
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| Authors: | M Zadworny M Guzicka P ?akomy S Samardakiewicz D J Smoliński J Mucha |
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| Institution: | 1. Institute of Dendrology, Polish Academy of Sciences, , 62‐035 Kórnik, Poland;2. Department of Forest Pathology, Faculty of Forestry, Poznań University of Life Sciences, , Poznań, Poland;3. Laboratory of Electron and Confocal Microscopy, Faculty of Biology, Adam Mickiewicz University, , Poznań, Poland;4. Department of Cell Biology, Institute of General and Molecular Biology, Nicolaus Copernicus University, , Toruń, Poland |
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| Abstract: | Cytoskeletal dynamics play a crucial role in pathogen recognition and cell defence during the initial interactions between an invader and plant host. The aim of the work reported here was to characterize how Heterobasidion annosum s.s., Heterobasidion parviporum, and Heterobasidion abietinum affect the microtubules and microfilaments of Pinus sylvestris root cells 12‐, 24‐, 48‐, and 96‐h post‐inoculation. Inoculation of P. sylvestris with H. parviporum or H. abietinum, which have a lower specificity for P. sylvestris than H. annosum s.s, resulted in greater reorganization of host microtubules during the early stages of interaction than inoculation with the more specific H. annosum s.s. In some infected cells, spots of actin aggregates were observed. Disruption of cytoskeletal components by the application of specific cytoskeletal inhibitors facilitated the entry of the H. parviporum and H. abietinum into roots. These results suggest that the P. sylvestris cytoskeleton plays a role in the host response in the initial stages of the host–pathogen interaction. |
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