Production of protocorm-like bodies with bioreactor and regeneration in vitro of Oncidium ‘Sugar Sweet’ |
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Authors: | JF Yang XC Piao D Sun ML Lian |
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Institution: | 1. Key Laboratory of Nature Resource of Changbai Mountain & Functional Molecular (Yanbian University), Ministry of Education, Park Road 977, Yanji, Jilin 133002, China;2. Division of Applied Plant Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea;3. College of Horticulture, Shenyang Agriculture University, Shenyang, Liaoning 110866, China |
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Abstract: | To produce mass propagules of Oncidium ‘Sugar Sweet’, we tested the feasibility of producing protocorm-like bodies (PLBs) using 5 l balloon type air-lift bioreactors, and selected an optimal culture medium for shooting and rooting in vitro. The results showed that liquid bioreactor cultures were more efficient for PLBs proliferation when compared to solid- and liquid-agitated flask cultures. The maximum PLBs biomass (326.3 g per bioreactor, FW) was obtained in the immersion bioreactor culture, with the growth ratio reaching 10.9 after 50 days of culture. This was obviously higher than the ebb and flood bioreactor culture. During bioreactor culture, sucrose and electric conductivity (EC) in the culture medium were negatively correlated with PLBs growth; the highest PLBs fresh and dry biomass was obtained 40 days after culture. An inoculation density of 20 g (FW) was optimal for PLBs growth in a 3 l working volume of 5 l bioreactor. Furthermore, MS medium supplemented with 2.0 mg l−1 BA for shooting and 0.5 mg l−1 IBA for rooting was optimal during in vitro culture, the plantlets were successfully established in the potting substrate. |
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Keywords: | BA benzyladenine DW dry weight EC electric conductivity FW fresh weight IBA indole-3-butyric acid MS Murashige and Skoog NAA α-naphthalene acetic acid PLBs protocorm like-bodies rpm revolutions per min vvm air volume/culture volume per min |
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