基于正交试验的春兰分株繁殖技术优化研究

为优化春兰分株繁殖技术,以NAA为处理试剂,以兰苗成活率为评价指标,采用单因素试验、正交试验优化春兰分株繁殖影响因素。结果表明：影响因素顺序为NAA浸泡时间＞浸泡浓度＞环境温度＞光照强度＞光照时间。根据正交试验K值可得,春兰分株繁殖影响因素最优组合为NAA浸泡浓度0.3mg/L、浸泡时间15min、环境温度21℃、光照强度1000Lx、光照时间10h/d。经验证在此优化因素下,春兰分株繁殖实际成活率高达82.76%。

Optimization of Spring Orchid Splitting PropagationTechnology Based on Orthogonal Experiments

In order to optimize the reproductive technology of spring orchids, NAA was used as the treatment reagent, and the survival rate of orchids was used as the evaluation index. Single factor experiments and orthogonal experiments were used to optimize the influencing factors of spring orchid reproductive. The results show that the order of influencing factors is NAA soaking time>soaking concentration>environmental temperature>light intensity>light time. According to the K value of the orthogonal experiment, the optimal combination of factors affecting the reproduction of spring orchids is NAA soaking concentration of 0.3mg/L, soaking time of 15min, environmental temperature of 21 ℃, light intensity of 1000Lx, and light time of 10h/d. After verification, under this optimization factor, the actual survival rate of spring orchid branch propagation is as high as 82.76%.