| 农杆菌介导的马铃薯块茎 GUS 基因瞬时表达研简 |
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| 引用本文： | 究 舒 锐,刘少军,岳林旭,焦 健,李 燕.农杆菌介导的马铃薯块茎 GUS 基因瞬时表达研简[J].天津农业科学,2013(11):1-3. |
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| 作者姓名： | 究 舒 锐 刘少军 岳林旭 焦 健 李 燕 |
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| 作者单位： | 山东省轻工农副原料研究所,山东高密261500 |
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| 摘 要： | 采用根癌农杆菌介导的瞬时表达方法,以马铃薯块茎为主要的研究材料,分别采用浸染法和注射法将外源基因导入马铃薯块茎细胞中,然后进行GUS组织化学染色.试验结果表明,导入马铃薯块茎细胞里的外源基因获得了表达；浸染法的表达区域更加均匀,优于注射法.
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| 关 键 词： | 马铃薯 块茎 根癌农杆菌 瞬时表达 GUS |
Research on the Transient Expression of GUS Gene by Agrobacterium tumefaciens in Potato Tubers |
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| SHU Rui,LIU Shao-jun,YUE Lin-xu,JIAO Jian,LI Yan.Research on the Transient Expression of GUS Gene by Agrobacterium tumefaciens in Potato Tubers[J].Tianjin Agricultural Sciences,2013(11):1-3. |
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| Authors: | SHU Rui LIU Shao-jun YUE Lin-xu JIAO Jian LI Yan |
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| Institution: | (Research Institute of Light Industry, Agriculture and Sideline of Shandong Province, Gaomi, Shandong 261500,China ) |
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| Abstract: | Agrobacterium tumefaciens as the transient expression agent and potato tubers were made use of the research materials. Impregnation method and injection method were used to introduce foreign gene into potato tuber cells.The GUS transient staining analysis indicated that the foreign gene was transplanted into the potato tuber cells successfully. And the impregnation method was better than the injection method. |
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| Keywords: | potato tuber Agrobacterium tumefaciens transient expression GUS |
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