一串红高效离体再生体系研究

为建立一串红高效再生体系,研究了0.1% HgCl2不同灭菌时间对一串红种子和外植体污染率和启动率(发芽率)的影响,以及不同激素组合对一串红外植体茎段、带节茎段和叶片愈伤组织诱导率及其分化率的影响。结果表明：采用0.1% HgCl2处理9 min是一串红外植体较为理想的表面灭菌时间,而一串红种子处理8 min有较低的污染率和较高的发芽率；采用无菌苗的茎段和叶片能诱导出质量较好的愈伤组织,但其进一步分化产生不定芽的能力较差,而采用带节茎段诱导愈伤获得了较多的分化苗,且1.5 mg ·L-1 6-BA+1.0 mg·L-1 2,4-D+0.2 mg ·L-1 NAA配方适于一串红愈伤组织的诱导,2.0 mg ·L-16-BA+0.1 mg ·L-1 NAA组合是适宜一串红愈伤组织分化的激素配方。此外,含0.5 mg ·L-1 NAA的1/2MS固体培养基能使一串红分化苗生根率在90%以上,且移栽后一串红成活率可达80%以上。

Elementary study on the high effective regeneration system of Salvia Splendens

In order to establish a high effective regeneration system of Salvia Splendens,the effects of different sterilization time of 0.1% HgCl2(mercuric chloride)on contamination rate and germination rate of seeds and explants including leaf segments,stem segments and stems segments with axillary bud,and the effects of different hormones on callus induction rate and callus differentiation rate of explants were studied.The results showed that 9 min with 0.1% HgCl2 was a ideal explants surface sterilization time,and...