| 分子标记辅助选择Xa23基因在选育抗白叶枯病水稻新品系中的应用 |
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| 引用本文： | 刘艳,王逸超,樊继伟,卢白关,秦德荣,曹文磊,王萍,徐大勇.分子标记辅助选择Xa23基因在选育抗白叶枯病水稻新品系中的应用[J].浙江农业学报,2011,23(2). |
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| 作者姓名： | 刘艳 王逸超 樊继伟 卢白关 秦德荣 曹文磊 王萍 徐大勇 |
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| 作者单位： | 1. 连云港市农业科学院,江苏连云港,222006
2. 淮海工学院海洋学院,江苏连云港,222005 |
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| 基金项目： | 国家水稻产业技术体系连云港综合试验站建设基金，农业科技攻关项目 |
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| 摘 要： | 以携带抗白叶枯病基因Xa23的抗病品系‘中野5112'为抗源,以优良水稻品种‘中稻88'和‘中粳Q-196'为受体材料,采用杂交和回交在分离群体中利用与Xa23紧密连锁的EST标记C189进行分子标记辅助选择,通过分子标记检测和成株期农艺性状选择,获得70份目标基因纯合且农艺性状稳定的株系.使用鉴别菌系P6,采用人工剪叶接种法,在孕穗期对44份重点株系进行抗性鉴定,所有株系在孕穗期都表现抗病.结果表明利用与Xa23基因紧密连锁的分子标记C189开展抗白叶枯病分子标记辅助育种是一简便、有效的途径.
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| 关 键 词： | 水稻 分子标记辅助选择 Xa23 白叶枯病 |
Application of marker-assisted selection of Xa23 gene in enhancing bacterial leaf blight resistance in rice |
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| LIU Yan,WANG Yi-chao,FAN Ji-wei,LU Bai-guan,QIN De-rong,CAO Wen-lei,WANG Ping,XU Da-yong.Application of marker-assisted selection of Xa23 gene in enhancing bacterial leaf blight resistance in rice[J].Acta Agriculturae Zhejiangensis,2011,23(2). |
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| Authors: | LIU Yan WANG Yi-chao FAN Ji-wei LU Bai-guan QIN De-rong CAO Wen-lei WANG Ping XU Da-yong |
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| Institution: | LIU Yan1,WANG Yi-chao2,FAN Ji-wei1,LU Bai-guan1,QIN De-rong1,WANG Ping2,XU Da-yong1(1Lianyungang Academy of Agricultural Sciences,Lianyungang 222006,China,2Huaihai Institute of Technology,School of Marine Science and Technology,Lianyungang 222005,China) |
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| Abstract: | The bacterial leaf blight(BB) resistant gene Xa23 from Zhongye 5112 was introduced into a restorer line Zhongdao 88 and Zhongjing Q-196,which is susceptible to BB by crossing and marker-assisted selection.The Xa23-linked EST marker C189 was used to select Xa23 in the off-spring.Marker-assisted selection(MAS) for Xa23 was performed at booting stages,and followed by conventional selection for agronomic traits at the ripening stage.More than 70 introgression lines that harbored homozygous Xa23 and showed stabl... |
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| Keywords: | rice(Oryza sativa L ) molecular marker-assisted selection(MAS) Xa23 rice bacterial leaf blight |
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