| 两个大豆灰斑病抗性相关SCAR标记的发现与鉴定 |
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| 引用本文： | 丁俊杰,文景芝,束永俊,李勇,柏锡,才华,纪巍,季佐军,朱延明.两个大豆灰斑病抗性相关SCAR标记的发现与鉴定[J].东北农业大学学报,2010,41(12). |
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| 作者姓名： | 丁俊杰 文景芝 束永俊 李勇 柏锡 才华 纪巍 季佐军 朱延明 |
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| 作者单位： | 黑龙江省农业科学院佳木斯分院,黑龙江,佳木斯,154007;东北农业大学农学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030 |
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| 基金项目： | 国家高技术研究发展计划"863计划"项目 |
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| 摘 要： | 大豆灰斑病是一种由大豆灰斑病菌引起的真菌性病害,会导致大豆叶片出现病斑、大豆籽粒斑驳,造成减产和品质下降,对大豆生产有重要影响。研究通过田间试验对142份育成大豆新品种(系)的灰斑病抗性进行鉴定,进而对大豆灰斑病抗性资源进行评估。利用前期开发的功能分子标记进行候选基因关联分析,筛选得到两个与大豆灰斑病抗性相关的SCAR标记,可用于大豆抗灰斑病的分子辅助育种研究。测序分析发现,它们定位于两个磷脂酶D基因内含子上,是由大豆转座子插入突变形成的。这两个SCAR标记具有功能基因靶向和操作简便等优点,具有广阔的大豆抗灰斑病育种应用前景。
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| 关 键 词： | 大豆灰斑病 SCAR标记 基因关联分析 磷脂酶D |
Discovery and identification of two SCAR related markers resistance to soybean frogeye leaf spot |
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| DING Junjie,WEN Jingzhi,SHU Yongjun,LI Yong,BAI Xi,CAI Hua,JI Wei,JI Zuojun,ZHU Yanming.Discovery and identification of two SCAR related markers resistance to soybean frogeye leaf spot[J].Journal of Northeast Agricultural University,2010,41(12). |
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| Authors: | DING Junjie WEN Jingzhi SHU Yongjun LI Yong BAI Xi CAI Hua JI Wei JI Zuojun ZHU Yanming |
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| Institution: | DING Junjie1,WEN Jingzhi2,SHU Yongjun3,LI Yong3,BAI Xi3,CAI Hua3,JI Wei3,JI Zuojun3,ZHU Yanming3(1.Jiamusi Branch Institute,Heilongjiang Academy of Agricultural Sciences,Jiamusi Heilongjiang 154007,China,2.College of Agriculture,Northeast Agricultural University,Harbin 150030,3.College of Life Sciences,China) |
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| Abstract: | Frogeye leaf spot(FLS) is caused by a fungal pathogen,Cercospora sojina K.Hara,which would lead the soybean leaf lesion and the soybean seed coat mottle.FLS is a common disease of soybean in most of the soybean-growing countries in the world,which would reduce the production of world soybean;decline the quality of soybean;and impact the soybean production in the world.Therefore,the resistance to frogeye leaf spot of soybean breeding cultivars(lines) was identified through field experiments in this study,and... |
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| Keywords: | soybean frogeye leaf spot(FLS) SCAR marker gene association analysis phospholi-pases D |
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