| 治疗奶牛乳腺炎蒙药复方提取物抗大肠埃希菌和金黄色葡萄球菌作用研究 |
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| 引用本文： | 包金荣,王纯洁,战永波,敖日格乐,吴海燕.治疗奶牛乳腺炎蒙药复方提取物抗大肠埃希菌和金黄色葡萄球菌作用研究[J].动物医学进展,2010,31(9). |
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| 作者姓名： | 包金荣 王纯洁 战永波 敖日格乐 吴海燕 |
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| 作者单位： | 内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特,010018 |
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| 基金项目： | 内蒙古自然科学基金重点项目，教育部春晖计划启动资金，内蒙古教育厅高校项目 |
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| 摘 要： | 对两种蒙药复方进行乙醇回流提取,然后分别用石油醚、乙酸乙酯、正丁醇萃取,得到石油醚层浸膏、乙酸乙酯层浸膏、正丁醇层浸膏和水母液浸膏,并分别对各种提取物进行奶牛乳腺炎两种主要致病菌大肠埃希菌和金黄色葡萄球菌的体外抑菌和体内抗菌作用,用牛津杯法测定提取物对大肠埃希菌和金黄色葡萄球菌抑菌圈直径,用二倍稀释法测定了其在体外的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。又以两种致病菌的混合菌感染小鼠,灌服蒙药复方提取物以测定其体内抗菌效果。结果表明,两种蒙药复方的体外抑菌成分主要集中在乙酸乙酯层浸膏和正丁醇层浸膏内,抑菌环直径均大于16.88 mm,最大达到21.61 mm;对两种致病菌的MIC和MBC最低可达到0.031 5 g/mL和0.062 5 g/mL;两种蒙药复方提取物的体内抗菌成分重要集中在石油醚层浸膏和水母液浸膏内,对小鼠死亡保护率明显高于阴性对照组,可达87.5%。
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| 关 键 词： | 蒙药 抑菌 小鼠 存活率 |
Effect of Compound Mongolian Medicine Extract Curing Cow Mastitis on Anti-Escherichia coli and Staphylococcus aureus |
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| BAO Jin-rong,WANG Chun-jie,ZHAN Yong-bo,AO Ri-ge-le,WU Hai-yan.Effect of Compound Mongolian Medicine Extract Curing Cow Mastitis on Anti-Escherichia coli and Staphylococcus aureus[J].Progress In Veterinary Medicine,2010,31(9). |
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| Authors: | BAO Jin-rong WANG Chun-jie ZHAN Yong-bo AO Ri-ge-le WU Hai-yan |
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| Institution: | BAO Jin-rong,WANG Chun-jie,ZHAN Yong-bo,AO Ri-ge-le,WU Hai-yan(College of Animal Science and Veterinary Medicine,Inner Mongolia Agriculture University,Huhhot,Inner Mongolia,010018,China) |
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| Abstract: | To study the antibacterial effect of compound Mongolian medicine extract on two main pathogenic bacteria,Staphylococcus aureus and Escherichia coli of cow mastitis,in vitro and in vivo,the bacteriostasis circle diameters of the exstracts were determined by Oxford cup law,the minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of compound Mongolian medicine extracts were examined in vitro by two times dilution method.Besides,the antibacterial effect of Mongolian medicine extract... |
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| Keywords: | Mongolian medicine anti-bacterium mouse survival rate |
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