包被肉桂油对1~21日龄肉鸡生长性能和回肠微生物区系的影响

为进一步探究肉桂油的应用效果，本试验在前期研究基础上探讨了饲粮中添加300 mg/kg包被肉桂油对肉鸡回肠菌群多样性和组成的影响。试验采用单因素设计，将132只1日龄科宝500肉鸡随机分成对照组和试验组，每个处理组6个重复，每个重复11只鸡（5只公鸡，6只母鸡）。对照组饲喂基础饲粮，试验组饲粮在基础饲粮中添加300 mg/kg包被肉桂油，试验期为21 d。结果显示，饲粮中添加300 mg/kg包被肉桂油对1~21日龄肉鸡生长性能无显著影响（P>0.05）。与对照组相比，试验组肉鸡21日龄回肠微生物的Shannon指数和Sobs指数显著增加（P<0.05），Simpson指数显著降低（P<0.05）。两组回肠微生物共有的OTU数为133个，对照组和试验组独有的OTU数分别为4和135个。ANOSIM分析显示，肉鸡回肠微生物的组间差异大于组内差异（R=0.4896）且差异显著（P<0.05）。试验组回肠链球菌属相对丰度显著低于对照组（P<0.05）；两组回肠其他属细菌丰度差异均不显著（P>0.05）。综上所述，饲粮中添加300 mg/kg包被肉桂油有利于增加21日龄肉鸡回肠微生物多样性，降低链球菌属细菌的相对丰度。

Effects of Coated Oleum cinnamomi Supplementation on Growth Performance and Ileal Microbiota of Broilers from 1 to 21 Days Old

In order to further explore the effective utilization of Oleum cinnamomi,based on the previous studies,the present study was conducted to investigate the effect of dietary addition of 300 mg/kg coated Oleum cinnamomi on microbial diversity and composition in the ileum of broiler chickens.Single factor experimental design was employed and a total of 132 one-day-old Cobb 500 broiler chickens were randomly assigned into control and experimental groups,respectively.There were 6 replicates in each group with 11 chickens (5 males and 6 females) in each replicate.The chickens in control group were fed the basal diet and the ones in experimental group were fed basal diet supplemented with 300 mg/kg of coated Oleum cinnamomi.The trail lasted for 21 d.The results showed that:Dietary addition of 300 mg/kg of coated Oleum cinnamomi did not affect growth performance (P>0.05).Compared with the control group,experimental group significantly increased Shannon and Sobs indices and significantly decreased the Simpson index (P<0.05).There were 133 shared OTUs in the ileal microflora of both control and experimental groups,4 unique OTUs in the control group,and 135 unique OTUs in the experimental group.The ANOSIM analysis showed that the inter-group difference of ileal microbiota composition was higher than that of intra-group (P<0.05).The relative abundance of Streptococcus spp.in the ileum of chickens in experimental group was lower than that in the control group (P<0.05).There was no significant difference in ileal abundance of other genus between two groups (P>0.05).In summary,dietary addition of 300 mg/kg of coated Oleum cinnamomi increased the microbial diversity,and reduced the relative abundance of Streptococcus spp.in the ileum of broiler chickens at 21 days of age.