乌梅散提取物对兔离体小肠平滑肌运动的影响及机制研究

本研究旨在探究乌梅散提取物对兔离体小肠平滑肌收缩的作用及其机制。取乌梅30 g、柿蒂50 g、诃子12 g、黄连12 g、郁金12 g，通过煎熬制备乌梅散水提取物储液。兔小肠离体后恒温灌流，利用RM6240多道生物信号采集处理系统进行信息采集和记录。观察乌梅散提取物对离体小肠自发收缩的影响，应用乙酰胆碱（Ach）致使兔小肠平滑肌痉挛性收缩，观察中浓度乌梅散提取物（2.18 g/L）对Ach诱导的收缩的影响，并观察其对Ach引起的离体小肠细胞内Ca²⁺和胞外Ca²⁺收缩的影响。试验结果表明，在药物浓度大于1.09 g/L时，可显著抑制兔离体小肠平滑肌自发收缩的振幅和频率（P<0.05）；Ach可极显著诱导小肠平滑肌收缩的振幅（P<0.01）；2.18 g/L乌梅散提取物可极显著抑制Ach引起的痉挛性收缩振幅和频率（P<0.01）；在无Ca²⁺台氏液中，乌梅散提取物可极显著抑制Ach引起的离体小肠细胞内Ca²⁺和胞外Ca²⁺收缩振幅及频率（P<0.01）。综上所述，乌梅散提取物剂量依赖性地抑制小肠平滑肌自发收缩，可抑制小肠痉挛性收缩，其机制可能与抑制细胞内Ca²⁺释放和胞外Ca²⁺内流有关。

Effects of the Dark Plum Powder Extraction on Contraction of Small Intestine Smooth Muscle of Rabbits in vitro and Its Mechanism

The effects of Dark Plum powder extraction on the contraction of small intestine in vitro were investigated and the possible mechanism was explored.The water extraction was prepared by decocting the following Chinese herbs including 30 g of Dark Plum,50 g of Calyx Kaki,12 g of Medicine Terminalia Fruit,12 g of Coptidis Rhizoma,and 12 g of Radix Curcumae.The technique of isothermal perfusion of small intestine in vitro was used.RM6240 multi-channel biological signal acquisition and processing system was used for information collection and processing.The effects of Dark Plum powder extraction on the spontaneous contraction of isolated gastrointestinal smooth muscles were observed.The effects of 2.18 g/L of Dark Plum powder extraction on the contraction induced by acetylcholine(Ach)were measured.The effects of 2.18 g/L of Dark Plum powder extraction on the Ach-induced both intracellular and extracellular calcium-depended contractions were studied.The amplitude and frequency of spontaneous contraction of isolated gastrointestinal smooth muscles were significantly reduced by Dark Plum powder extraction at the concentration of more than 1.09 g/L(P<0.05).Ach observably induced the amplitude of smooth muscle contractions(P<0.01).Dark Plum powder extraction at the concentration of 2.18 g/L markedly inhibited the amplitude and frequency of spasmodic contractions(P<0.01).In the Ca²⁺free tyrodes solution,Dark Plum powder extraction evidently inhibited the amplitude and frequency of the Ach-induced both intracellular and extracellular calcium-depended contractions(P<0.01).Dark Plum powder extraction inhibited the spontaneous contraction and spasmodic contraction caused by Ach in a dose-dependent manner,isolated gastric smooth muscle.The mechanism was speculated to be related to inhibiting the extracellular Ca²⁺inflowing via cell membrane and intracellular Ca²⁺releasing.