绒山羊皮肤毛囊兴盛前期组织形态、chi-miR-107-3p与RHBDF2基因表达分析

为了探究chi-miR-107-3p和菱形家族蛋白2（rhomboid family member 2，RHBDF2）基因在不同品种（系）和光控增绒绒山羊兴盛前期（5~7月份）皮肤毛囊重建时的表达差异，本试验在7月份采集内蒙古阿拉善型绒山羊、敏盖绒山羊和光控增绒技术处理的阿尔巴斯型绒山羊体侧皮肤毛囊组织，采用组织切片技术对组织形态比较分析，实时荧光定量PCR法检测chi-miR-107-3p和RHBDF2基因的表达量。结果显示，在皮肤毛囊兴盛前期，阿拉善型绒山羊次级毛囊正处于重建阶段，敏盖绒山羊和阿尔巴斯型绒山羊（光控增绒）次级毛囊重建已基本完成；在阿拉善型绒山羊皮肤组织中chi-miR-107-3p表达量极显著高于敏盖绒山羊和阿尔巴斯型绒山羊（光控增绒）（P<0.01），而RHBDF2基因表达量极显著低于其他两品种（系）（P<0.01），且RHBDF2基因表达量在敏盖绒山羊皮肤组织中显著低于阿尔巴斯型绒山羊（P<0.05）。不同品种（系）和光控增绒绒山羊皮肤毛囊兴盛前期组织显微结构与chi-miR-107-3p、RHBDF2基因在皮肤组织中的表达差异分析结果相一致，次级毛囊重建初期chi-miR-107-3p表达量较高，而RHBDF2基因表达量极低，随着次级毛囊重建完成，chi-miR-107-3p表达量明显降低，RHBDF2基因表达量逐渐增高，且在不同品种（系）内蒙古绒山羊绒毛生长发育过程中的表达机制基本相同。因此，chi-miR-107-3p和RHBDF2基因是绒山羊皮肤毛囊生长发育的重要调控因子。

Expression Analysis of Histomorphology,chi-miR-107-3p and RHBDF 2 Genes of Hair Follicle in Cashmere Goats at Anagen Prosperity

The aim of this study was to explore the effect of different expressions of chi-miR-107-3p and rhomboid family member 2(RHBDF2) genes on the villus growth of cashmere goats in different breeds (strains) and light controlled cashmere increasing in the skin hair follicle anagen (May to July).In this experiment,the hair follicle tissues of Inner Mongolia Alashan cashmere goats,Mingai cashmere goats and Albas cashmere goats which were used the increasing cashmere yield technology by shortened photoperiod were randomly collected in July,and HE staining was used to observe the morphology differences.Real-time quantitative PCR was used to detect the ralative expression of chi-miR-107-3p and RHBDF2 genes.The results showed that the secondary hair follicles of Alashan cashmere goats were in the stage of growth and development,and the reconstruction of secondary hair follicles of Mingai cashmere goats and Albas cashmere goats (light controlled cashmere increasing) had been basically completed.The expression of chi-miR-107-3p in the skin tissue of Alashan cashmere goats was extremely significantly higher than that of Mingai cashmere goats and Albas cashmere goats (P<0.01),the expression level of RHBDF2 gene was extremely significantly lower than that of the other two breeds (P<0.01),but the expression level of RHBDF2 gene in skin tissue of Mingai cashmere goats was significantly lower than that of Albas cashmere goats (P<0.05).In conclusion,the microstructure of hair follicle in different breeds (strains) light controled cashmere goats at anagen prosperity was consistent with the results of the differential analysis of chi-miR-107-3p and RHBDF2 genes in skin tissues.The expression level of chi-miR-107-3p was high,while the expression level of RHBDF2 gene was extremely low.With the completion of secondary hair follicle reconstruction,the expression of chi-miR-107-3p decreased significantly,the expression of RHBDF2 gene increased gradually,and the expression mechanism of Inner Mongolia cashmere in different breeds (strains) was basically the same.chi-miR-107-3p and RHBDF2 genes were important regulators for the growth and development of skin hair follicles in cashmere goats.