基于双向电泳技术研究湖羊初乳和常乳乳蛋白质组差异

试验旨在分析绵羊初乳和常乳乳蛋白质组的差异，揭示绵羊不同泌乳阶段的乳蛋白质组特征。选用6只胎次相同、预产期相近的湖羊母羊，分别采集分娩后第1、3、7天的初乳和第14、28、56天的常乳，每个时间点采集6份奶样并等体积混合，离心弃去乳脂肪，采用双向电泳（2-DE）技术对初乳和常乳的脱脂乳蛋白进行比较分析。结果发现，初乳中乳蛋白含量较高，且随着泌乳期的延伸，乳蛋白含量降低。以第1天乳蛋白2-DE图谱为参照，第3天检测到38个差异蛋白点，其中有20个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第3天图谱中，其余17个蛋白点呈现表达量的差异；第7天检测到35个差异蛋白点，其中有19个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第7天图谱中，其余15个蛋白点呈现表达量的差异；第14天检测到34个差异蛋白点，其中有11个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第14天图谱中，其余22个蛋白点呈现表达量的差异；第28天检测到38个差异蛋白点，其中有28个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第28天图谱中，其余9个蛋白点呈现表达量的差异；第56天检测到36个差异蛋白点，其中有26个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第56天图谱中，其余9个蛋白点呈现表达量的差异。综上，共检测出44个差异表达的蛋白点，其中有8个蛋白点仅存在于第1天图谱中，而这些蛋白主要涉及免疫活性功能。

Differential Proteomic Study of Colostrum and Mature Milk of Hu Sheep Based on Two-dimensional Electrophoresis Technology

The objective of this experiment was to analyze the differential proteomic between colostrum and mature milk of sheep,and provide further insight into the proteome characteristics during different lactation periods.6 Hu sheep with the same parity and similar due date were selected to collect colostrum on the 1st,3rd,7th day and mature milk on the 14th,28th,56th day postpartum.6 units of milk were mixed as a tested sample with equal volume at each time point,and defatted milk was prepared by removing the fat from the whole milk.The defatted milk of colostrum and mature milk were analyzed by two-dimensional electrophoresis (2-DE).The results showed that the content of milk protein was higher in colostrum,and decreased with the extension of lactation stage.According to the 2-DE map of milk protein on the 1st day,38 different protein spots were detected on the 3rd day,20 of which only existed on the 1st day and 1 only existed on the 3rd day,the other 17 protein spots showed differential expression.On the 7th day,35 different protein spots were detected,19 of which only existed on the 1st day and 1 only existed on the 7th day,the other 15 protein spots showed differential expression.On the 14th day,34 different protein spots were detected,11 of which only existed on the 1st day and 1 only existed on the 14th day,the other 22 protein spots showed differential expression.On the 28th day,38 different protein spots were detected,28 of which only existed on the 1st day and 1 only existed on the 28th day,the other 9 protein spots showed differential expression.On the 56th day,36 different protein spots were detected,26 of which only existed on the 1st day and 1 only existed on the 56th day,the other 9 protein spots showed differential expression.To sum up,a total of 44 differentially expressed protein spots were detected,8 of which only existed on the 1st day of postpartum,and these proteins were mainly involved in immunological activity.