运输应激对马血细胞和血液生化指标的影响

为了调查运输应激对伊犁马血细胞和血液生化指标的影响，试验选取6匹伊犁母马，进行400 km里程的运输，总运输时间为8 h，分别在运输0、4、8 h和落地12 h后，测定马血清急性期蛋白（APPs）、皮质醇（Cor）、热休克蛋白（HSPs）、干扰素-γ（IFN-γ）和肿瘤坏死因子-α（TNF-α）含量以及血液常规指标。结果显示，与运输0 h相比，运输8 h马触珠蛋白（HP）含量显著上升（P<0.05），运输4、8 h和落地12 h后马血清Cor含量均极显著上升（P<0.01）；落地12 h后马血清HSP90含量极显著下降（P<0.01）；运输对马血清IFN-γ和TNF-α含量均无显著影响（P>0.05）；运输4、8 h和落地12 h后血液红细胞分布宽度标准差（RDW-SD）、血小板分布宽度（PDW）、血小板大细胞比率（P-LCR）、粒细胞（GRAN）和粒细胞比率（GRAN%）均显著上升（P<0.05），中间细胞比率（MID%）、中间细胞（MID）、血红蛋白浓度（MCHC）均显著下降（P<0.05）。综上所述，运输应激对马血清HP、Cor、HSP90浓度、血液RDW-SD、PDW、P-LCR、GRAN%、GRAN、MID%、MID和MCHC均产生显著影响，这些指标可用于评价马对运输应激的敏感性。

Effect of Transportation Stress on Horse Blood Cells and Blood Biochemical Indexes

In order to investigate the impact of transportation stress on blood cells and blood biochemical indicators of Ili horses,6 Ili mares were selected for transportation with a mileage of 400 km.The total transportation time were 8 h,the horse serum acute phase proteins(APPs),cortisol(Cor),heat shock proteins(HSPs),interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α)and blood routine were detected at 0,4,8 h of transport and 12 h after landing.The results showed that when compared with 0 h of transport,horses serum haptoglobin(HP)content increased significantly at 8 h of transport(P<0.05),horses serum Cor content increased significantly at 4 and 8 h of transport and 12 h after transport(P<0.01).Horses serum HSP90 content decreased significantly at the 12 h after landing(P<0.01),but transportation had no significant effect on serum IFN-γand TNF-αcontent(P>0.05).The standard deviation in red cell distribution width(RDW-SD),platelet distribution width(PDW),platelet large cell ratio(P-LCR),granulocyte(GRAN)and granulocyte ratio(GRAN%)were all significantly increased at 4 and 8 h of transport and 12 h after transport(P<0.05),and intermediate cells ratio(MID%),intermediate cells(MID),and hemoglobin concentration(MCHC)were all significantly decreased at 4 and 8 h of transport and 12 h after transport(P<0.05).In conclusion,transportation stress had significant effects on horses serum HP,Cor,HSP90 concentration,blood RDW-SD,PDW,P-LCR,GRAN%,GRAN,MID and MCHC,these indicators could be used to evaluate horses sensitivity to transportation stress.