牦牛源溶血致病性表皮葡萄球菌的分离鉴定及药敏分析

对1株分离自西藏那曲地区养殖场有出血性症状牦牛的致病性菌进行分子鉴定及药敏分析，为西藏地区牦牛出血性疾病提供治疗依据。通过对病死牦牛肺脏、肝脏进行细菌分离纯化获得疑似菌株，对所得疑似菌株进行形态学观察与哥伦比亚血平板试验筛选出疑似致病菌株，再对疑似致病菌株进行生理生化鉴定试验、16S rDNA通用引物检测及测序、同源性比对，后经药敏试验得到该疑似致病菌株的敏感药物，最后通过动物回归试验验证其敏感药物的治疗效果。结果表明，通过对病死牦牛肺脏、肝脏分离纯化获得6株疑似菌株，经形态学观察试验、哥伦比亚血平板试验筛选出1株具有溶血性的革兰氏阳性球菌S-4。经生理生化鉴定试验、16S rDNA测序、同源性比对，鉴定S-4菌株为表皮葡萄球菌；药敏试验结果显示，S-4菌株对恩诺沙星、新霉素、多黏菌素B、卡那霉素、环丙沙星敏感，对氟苯尼考、多西环素中介敏感，对链霉素、红霉素、四环素、青霉素、头孢氨苄耐受；动物回归试验显示，该菌株具有致病性，且恩诺沙星、新霉素、卡那霉素3种药物治疗效果良好，多黏菌素B、环丙沙星治疗效果差。本试验获得1株具有致病性的牦牛源表皮葡萄球菌，该致病菌在养殖过程中可使用恩诺沙星、新霉素、卡那霉素3种药物进行治疗。

Isolation,Identification and Drug Sensitivity Analysis of Remolytic Pathogenic Staphylococcus epidermidis from Yak

Molecular identification and drug susceptibility of a strain pathogenic bacteria isolated from a yak suffered from bleeding from a farm of Naqu city,Tibet,were analyzed,in order to provide treatment bases for hemorrhagic disease of yaks in Tibet.The suspected strains were obtained by bacterial isolation and purification from lungs and livers of dead yaks,then the suspected strains were screened out by morphological observation and Columbia blood plate test,and then physiological and biochemical tests were done,16S rDNA sequencing and homology comparison test on the obtained suspected strains,and then obtained sensitive drugs of the suspected pathogenic strains by drug sensitivity tests,finally verified treatment effect of the sensitive drugs by animal regression test.The results showed that 6 suspected strains were obtained by separation and purification of the lungs and livers of dead yaks,and a strain of hemolytic Gram-positive cocci S-4 was screened out by morphological observation test and Columbia blood plate test,and the S-4 strain was identified as Staphylococcus epidermidis by the physiological and biochemical identification test,16S rDNA sequencing,homology comparison test.The drug sensitivity test showed that the S-4 strain was sensitive to enrofloxacin,neomycin,polymyxin B,kanamycin,and ciprofloxacin.And it was medium sensitive to florfenicol and doxycycline.And it was tolerant to streptomycin,erythromycin,tetracycline,penicillin and cephalexin.The animal regression tests showed that the strain was pathogenic and enrofloxacin,neomycin,and kanamycin,the 3 drugs had good treatment effects,while polymyxin B and ciprofloxacin had poor treatment effects.The results showed that this experiment obtained a pathogenic Staphylococcus epidermidis from yaks,the pathogenic bacteria could be treated with enrofloxacin,neomycin,and kanamycin during the breeding.