| Relationship between rooting ability and endogenous phytohormone changes in successive continuous generation cuttings of Buxus sinica var. parvifolia, an endangered woody species in China |
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| 作者姓名： | Huang Yan Ji Kong-shu Zhai Jin-ru |
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| 作者单位： | [1]Key Laboratory of Forest Genetics ＆ Gene Engineering of State Forestry Administration, Nanjing Forestry University, Nanjing 210037, P. R. China [2]Haoyu Landscape Co., Ltd, Rugao, Jiangsu 226553, E R. China |
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| 摘 要： | The objectives of this study were to investigate the effects of successive continuous generation (SCG) cuttings of Buxus sinica var. parvifolia on the rejuvenation of ortets at the hormone level, detect levels of indoleacetic acid (IAA), abscisic acid (ABA), isopentenyladenosine (iPA), zeatin riboside (ZR) and gibberellin4 (GA4) during the rooting process of different generations by means of enzyme-linked immunosorbent assay (ELISA) technology and statistically analyze the rooting characteristics of cuttings. The results show that, the root systems of subsequent generations (generation 1998, 2000) developed satisfactorily, only a few initial gen-eration (generation 1990, 1994) cuttings rooted and the root growth was inhibited. Around the period of callus formation and root projection through the epidermis, there was a marked, regular variation in the levels of IAA, ABA and GA4 between the initial gen-erations and subsequent generations.
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| 关 键 词： | 内生植物激素 复壮 中国 生根力 吲哚乙酸 玉米素核糖甙 |
| 收稿时间： | 25 October 2006 |
| 修稿时间： | 2006-10-25 |
Relationship between rooting ability and endogenous phytohormone changes in successive continuous generation cuttings of <Emphasis Type="Italic">Buxus sinica</Emphasis> var. <Emphasis Type="Italic">parvifolia</Emphasis>, an endangered woody species in China |
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| Huang Yan Ji Kong-shu Zhai Jin-ru.Relationship between rooting ability and endogenous phytohormone changes in successive continuous generation cuttings of Buxus sinica var. parvifolia, an endangered woody species in China[J].Forestry Studies in China,2007,9(3):189-197. |
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| Authors: | Huang Yan Ji Kong-shu Zhai Jin-ru |
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| Institution: | 1. Key Laboratory of Forest Genetics & Gene Engineering of State Forestry Administration, Nanjing Forestry University, Nanjing 210037, P. R. China
2. Haoyu Landscape Co., Ltd, Rugao, Jiangsu 226553, P. R. China |
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| Abstract: | The objectives of this study were to investigate the effects of successive continuous generation (SCG) cuttings of Buxus sinica var. parvifolia on the rejuvenation of ortets at the hormone level, detect levels of indoleacetic acid (IAA), abscisic acid (ABA), isopentenyladenosine (iPA), zeatin riboside (ZR) and gibberellin4 (GA4) during the rooting process of different generations by means of enzyme-linked immunosorbent assay (ELISA) technology and statistically analyze the rooting characteristics of cuttings. The results show that, the root systems of subsequent generations (generation 1998, 2000) developed satisfactorily, only a few initial gen-eration (generation 1990, 1994) cuttings rooted and the root growth was inhibited. Around the period of callus formation and root projection through the epidermis, there was a marked, regular variation in the levels of IAA, ABA and GA4 between the initial gen-erations and subsequent generations. |
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| Keywords: | Buxus sinica var parvifolia phytohormones successive continuous generation cutting rejuvenation |
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