Lipid biosynthesis from [14C]-glucose in the coral Montipora digitata |
| |
Authors: | Hirosuke OKU Hideyuki YAMASHIRO AND Kyoko ONAGA |
| |
Institution: | Division of Molecular Biotechnology, Center of Molecular Bioscience, University of the Ryukyus, Nishihara, Okinawa 903-0213 and ;Department of Tourism, Meio University, Nago, Okinawa 905-8585, Japan |
| |
Abstract: | ABSTRACT: Glucose has been implicated in functioning as a form of carbon translocated from symbiont zooxanthellae to the host coral cell. The present paper describes the lipid biosynthesis from 14C]-glucose in the coral tissue. To study the incorporation of 14C]-glucose into lipids, the branch tips of the coral Montipora digitata were incubated with 14C]-glucose or another radiolabeled substrates. The lipid biosynthesis from 14C]-glucose was dependent on light, and was decreased by dark conditions or by photosystem II inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Of the lipid classes, the light dependency was more pronounced with the biosynthesis of triacylglycerols (TG) and wax compared with phospholipids. Examination of 14C]-label distribution in the glycerolipids suggested that 14C]-glucose supplied mainly the fatty acid moiety of newly synthesized TG, while 14C]-glucose provided evenly the fatty acid moiety and the glycerol skeleton of phospholipids. The comparison of 14C]-labeling of lipid from host coral tissue and symbiont zooxanthellae suggested that 14C]-glucose entered the coral cell and was processed in parallel in the zooxanthellae and host cells. Furthermore, the coral cells used various 14C]-labeled sugars for lipid synthesis with similar lipid labeling profile as was the case for glucose. The current study thus supports the view that the low-molecular-weight compound, sugars and amino acids, once translocated from zooxanthellae to host cell were metabolized toward lipogenesis as well as glycerol. |
| |
Keywords: | coral glucose lipid biosynthesis photosynthesis sugars |
|
|