| 猪流感病毒RT-PCR检测方法的建立及初步应用 |
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| 引用本文： | 王隆柏,车勇良,吴学敏,陈如敬,刘玉涛,周伦江.猪流感病毒RT-PCR检测方法的建立及初步应用[J].福建农业学报,2014(7):618-622. |
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| 作者姓名： | 王隆柏 车勇良 吴学敏 陈如敬 刘玉涛 周伦江 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心,福建福州350013 |
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| 基金项目： | 福建省科技计划项目（2009Ri0025-5）;福建省现代农业（生猪）产业技术体系建设专项（2009） |
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| 摘 要： | 根据GenBank发表H1N1、H1N2和H3N2亚型猪流感病毒M基因片段的引物序列，设计合成1对引物，建立猪流感病毒RT-PCR检测方法。结果表明：建立的方法能扩增出675bp的基因片段，同条件扩增伪狂犬病毒、猪瘟病毒、圆环病毒Ⅱ型及猪繁殖与呼吸综合征病毒均为阴性；该方法可检测到3.5pg猪流感的核酸，能从被流感病毒感染的小白鼠和疑似猪流感的病料中检测到猪流感病毒。结果表明，建立的猪流感病毒RT-PCR方法具有特异性强、敏感性高和重复性好等特点，适用于对H1N1、H1N2和H3N2亚型猪流感病毒的快速诊断。
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| 关 键 词： | 猪流感病毒 RT-PCR 建立 |
The Establishment and Preliminary Application of a RT-PCR for Detection of Swine Lnfluenza Virus |
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| WANG Long-bai,CHE Yong-liang,WU Xue-min,CHEN Ru-jing,LIU Yu-tao,ZHOU Lun-jiang.The Establishment and Preliminary Application of a RT-PCR for Detection of Swine Lnfluenza Virus[J].Fujian Journal of Agricultural Sciences,2014(7):618-622. |
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| Authors: | WANG Long-bai CHE Yong-liang WU Xue-min CHEN Ru-jing LIU Yu-tao ZHOU Lun-jiang |
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| Institution: | (Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/ Animal Disease Control Technology Development Center, Fuzhou, Fujian 350013, China) |
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| Abstract: | A pair of primers was designed based on the M gene sequence of the Swine influenza virus （SIV） published in Genbank ,and the matched RT-PCR assay was developed . The 675 bp specific fragment was amplified from H1N1 ,H1N2 and H3N2 subtype Swine influenza viruses .Negative results were found on PRV ,CSFV ,PCV2 and PRRSV .The developed method was sensitive to detect RNA as little as 3.5 pg ,and Siv could be detected directly from lungs of infected mice and pathogenic swine . This RT-PCR assay was proved to be specific , sensitive , reproducible and suitable for rapid diagnosis of H 1N1 ,H1N2 and H3N2. |
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| Keywords: | swine Influenza virus RT-PCR establishment |
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