| 危害马铃薯的茎线虫分离鉴定 |
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| 引用本文： | 郭全新,简恒.危害马铃薯的茎线虫分离鉴定[J].植物保护,2010,36(3):117-120. |
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| 作者姓名： | 郭全新 简恒 |
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| 作者单位： | 中国农业大学植物病理系,北京,100193 |
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| 基金项目： | 公益性行业科研专项，农业部"948"项目，国家"十一五"科技支撑计划项目 |
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| 摘 要： | 本文对采自河北张家口市察北区、危害马铃薯的线虫(编号De-Chabei)进行了形态及分子生物学鉴定,形态测量结果表明其与马铃薯腐烂茎线虫(Ditylenchus destructor Thorne)一致,采用通用引物rDNA1/rDNA2扩增rD-NA-ITS区的长度为1130bp,测序比对以及采用马铃薯茎线虫B型特异性引物DdL1/DdL2扩增得到485bp特异性片段,表明与B型或L型马铃薯腐烂茎线虫一致;再次证明该型线虫在河北马铃薯主产区的分布和危害。
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| 关 键 词： | 马铃薯 马铃薯腐烂茎线虫 形态鉴定 ITS区分析 |
Identification of Ditylenchus destructor from potatoes in Zhangjiakou,Hebei |
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| Guo Quanxin,Jian Heng.Identification of Ditylenchus destructor from potatoes in Zhangjiakou,Hebei[J].Plant Protection,2010,36(3):117-120. |
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| Authors: | Guo Quanxin Jian Heng |
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| Institution: | Department of Plant Pathology, China Agricultural University, Beijing 100193, China |
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| Abstract: | A nematode population, named De Chabei, was isolated from potato in the Chabei District of Zhangjiakou City, Hebei Province. It had been identified as type B or L of Ditylenchus destructor Thorne based on morphological and molecular methods. The amplified products of rDNA ITS regions with the universal primers rDNA1/rDNA2 and the specific primers DdL1/DdL2 were 1 130 bp (GenBank accession No. FJ911551) and 485 bp, respectively. The results revealed that this type of D. destructor has existed in the potatogrowing areas of Hebei province. |
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| Keywords: | potato Ditylenchus destructor morphological identification ITS sequence analysis |
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