| 牛体细胞核移植方法研究 |
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| 引用本文： | 贾瑞贞,李煜,杨利军,黄翔华. 牛体细胞核移植方法研究[J]. 华北农学报, 2008, 23(5) |
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| 作者姓名： | 贾瑞贞 李煜 杨利军 黄翔华 |
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| 作者单位： | 内蒙古大学,哺乳动物生殖生物学与生物技术教育部重点实验室,内蒙古,呼和浩特,010021;内蒙古大学,哺乳动物生殖生物学与生物技术教育部重点实验室,内蒙古,呼和浩特,010021;内蒙古大学,哺乳动物生殖生物学与生物技术教育部重点实验室,内蒙古,呼和浩特,010021;内蒙古大学,哺乳动物生殖生物学与生物技术教育部重点实验室,内蒙古,呼和浩特,010021 |
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| 基金项目： | 国家自然科学基金资助项目，内蒙古自治区自然科学基金项目 |
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| 摘 要： | 研究了牛体细胞核移植过程的去核方法、融合条件等对重构胚发育的影响,旨在探索一种简化的核移植操作程序.结果表明,添加FSH,LH,最高成熟率为81.7%.在电压为2.6 kV/cm时,融合率为93.96%.激活后重构胚在压制的WOW内进行培养,采用半卵法去核的卵裂率和8-细胞发育率分别为61.2%和3.27%,盲切法去核的卵裂率和8-细胞发育率分别是 70.8%和7.56%.
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| 关 键 词： | 体细胞 核移植 徒手克隆 牛 |
Study on Bovine Somatic Cell Nuclear Transfer |
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| JIA Rui-zhen,LI Yu,YANG Li-jun,HUANG Xiang-hua. Study on Bovine Somatic Cell Nuclear Transfer[J]. Acta Agriculturae Boreali-Sinica, 2008, 23(5) |
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| Authors: | JIA Rui-zhen LI Yu YANG Li-jun HUANG Xiang-hua |
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| Affiliation: | JIA Rui-zhen,LI Yu,YANG Li-jun,HUANG Xiang-hua (Key laboratory of Mammal Reproductive Biology , Biotechnology,Ministry of Education,Inner Mongolia University,Huhhot 010021,China) |
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| Abstract: | The research was designed to explore a brief procedure on nuclear transfer,which included compare of different methods for mature culture of bovine occytes,separation and general culture of fibroblast taken from the skin of bovine,enucleated method and condition on fusion which exactly affect the development of reconstructed embryos.Finally the highest mature rate was 81.7% with FSH and LH added,and fusion rate was 93.96% with voltage of 2.6 kV/cm.The cleavage rate and 8-cell development rate of embryos the... |
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| Keywords: | Somatic cell Nuclear transfer Handmade cloning Bovine |
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