| mLIF、bFGF和SCF对鸡精原干细胞体外增殖的影响 |
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| 引用本文： | 魏彩霞,孙思宇,孙国波,赵文明,李碧春.mLIF、bFGF和SCF对鸡精原干细胞体外增殖的影响[J].中国畜牧杂志,2007,43(17):12-15. |
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| 作者姓名： | 魏彩霞 孙思宇 孙国波 赵文明 李碧春 |
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| 作者单位： | 1. 扬州大学动物科学与技术学院,江苏扬州,225009
2. 温州农业科学研究院,浙江温州,325006 |
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| 摘 要： | 采用MTT法分别检测mLIF、bFGF和SCF 3种细胞因子在单独添加和联合使用时对体外培养条件下的SSCs增殖的影响。结果表明:①3种细胞因子分别单独添加时,各实验组增殖效应与对照组比较差异显著(P<0.05);②3种因子联合使用时,低剂量组合共同促进SSCs的增殖,高剂量组合则抑制SSCs的增殖;③SCF、bFGF和mLIF的适宜剂量分别为10～20、10～20和10～15 ng/mL。
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| 关 键 词： | 精原干细胞 MTT 细胞因子 鸡 |
| 文章编号： | 0258-7033（2007）17-0012-04 |
| 修稿时间： | 2006-08-22 |
Effect of Different Cell Factors on Proliferation of Chicken Spermatogonial Stem Cells |
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| WEI Cai-xia,SUN Si-yu,SUN Guo-bo,ZHAO Wen-ming,LI Bi-chun.Effect of Different Cell Factors on Proliferation of Chicken Spermatogonial Stem Cells[J].Chinese Journal of Animal Science,2007,43(17):12-15. |
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| Authors: | WEI Cai-xia SUN Si-yu SUN Guo-bo ZHAO Wen-ming LI Bi-chun |
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| Institution: | 1.College of Animal Science and Technology, Yangzhou University, Jiangsu Yangzhou 225009, China; 2.Wenzhou Academy of Agrieuhural Sciences, Zhejiang Wenzhou 325006, China |
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| Abstract: | The effect of exogenous cell factors as mLIF,SCF and bFGF on SSCs proliferation and differentiation in vitro were elucidated by method of MTT test.The results showed that,there were significant differences between the experimental groups and control group as single cell factor was added to medium respectively(P < 0.05).When the cell factors as mLIF,SCF,but bFGF conbined in the medium,the proliferation of SSCs was enhanced at low concentration and inhibited at high concentration.The suitable concentration of SCF,bFGF and mLIF was 15~20,15~20 and 10~15 ng/mL,respectively. |
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| Keywords: | MTT |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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