| 嗜卷书虱和嗜虫书虱酯酶性质的比较研究 |
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| 引用本文： | 程伟霞,王进军,王梓英,赵志模,丁伟.嗜卷书虱和嗜虫书虱酯酶性质的比较研究[J].农药学学报,2002,4(4):61-66. |
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| 作者姓名： | 程伟霞 王进军 王梓英 赵志模 丁伟 |
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| 作者单位： | 1.西南农业大学 植物保护学院,昆虫学及害虫控制工程重点实验室,重庆 400716 |
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| 基金项目： | 国家自然科学基金(39800017)和霍英东基金(71022)资助项目. |
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| 摘 要： | 对嗜卷书虱Liposcelis bostrychophila和嗜虫书虱L. entomophila羧酸酯酶及乙酰胆碱酯酶(AChE)的生物化学性质进行了初步研究。结果表明:嗜虫书虱的羧酸酯酶活性明显高于嗜卷书虱,嗜卷书虱和嗜虫书虱羧酸酯酶对底物α-NA的Km值分别为0.665 7、0.285 7 mmol/L,Vmax值分别为1.672 2和2.463 1 mmol·(mg·30 min)-1。嗜卷书虱和嗜虫书虱乙酰胆碱酯酶的活性差异不显著,嗜虫书虱略高于嗜卷书虱。通过聚丙烯酰胺凝胶电泳,检测出8条嗜卷书虱、7条嗜虫书虱酯酶同工酶谱带,嗜卷书虱的酶谱分布比嗜虫书虱广。
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| 关 键 词： | 嗜卷书虱 嗜虫书虱 羧酸酯酶 乙酰胆碱酯酶 同工酶分析 |
| 文章编号： | 1008-7303(2002)04-0061-06 |
Comparison of the Activity of Carboxylesterase and Acetylcholinesterase in Liposcelis bostrychophila and L. entomophila |
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| CHENG Wei-xi,WANG Jin-jun,WANG Zi-ying,ZHAO Zhi-mo and DING Wei.Comparison of the Activity of Carboxylesterase and Acetylcholinesterase in Liposcelis bostrychophila and L. entomophila[J].Chinese Journal of Pesticide Science,2002,4(4):61-66. |
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| Authors: | CHENG Wei-xi WANG Jin-jun WANG Zi-ying ZHAO Zhi-mo and DING Wei |
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| Institution: | 1.College of Plant Protect, Southwest Agricultural University, Chongqing 400716, China |
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| Abstract: | The characteristics of carboxylesterase (CarE) and acetylcholinesterase (AChE) were investigated in Liposcelis bostrychophila and L. entomophila. The activity of CarE in L. entomophila was significantly higher than that in L. bostrychophila. The comparison of Michaelis constants (Km) of CarE between L. entomophila and L. bostrychophila showed that the affinity of the former to substrate-α-NA was much stronger than that of the latter. The activity of AChE in the two insects was similar. Totals of 8 and 7 esterase bands were detected on PAGE for L. bostrychophila and L. entomophila, respectively. And the esterase zymogram of L. bostrychophila was wider than that of L. entomophila. |
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| Keywords: | Liposcelis bostrychophila L entomophila acetylcholinesterase carboxylesterase isoenzyme |
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