试验用和临床用紫杉醇诱导肝癌细胞凋亡的比较

目的]为了探讨试验用和临床用紫杉醇对肝癌HepG2细胞凋亡诱导作用的差异。方法]用不同浓度的试验用和临床用紫杉醇处理肝癌HepG2培养细胞后,通过显微观察和流式细胞术检测其细胞凋亡。结果]临床用紫杉醇在终浓度为0.5、1.0和2.0 mg/ml时,24 h非常显著性诱导细胞凋亡,48 h诱导细胞凋亡更剧烈。试验用紫杉醇24 h在终浓度为2.5、5.0和10.0 mg/ml时,非常显著诱导细胞凋亡;当试验用紫杉醇浓度为2.5 mg/ml时,48 h诱导细胞凋亡更剧烈。当试验用紫杉醇浓度为5和10 mg/ml细胞凋亡率逐步变低,可能走向死亡。结论]在诱导肝癌细胞凋亡中,试验用紫杉醇要比临床所用紫杉醇的浓度要高。

Comparisons of Experimental or Clinically Used Paclitaxel in Inducing Hepatoma Cell Apoptosis

Objective] In order to explore the difference of experimental or clinically used paclitaxel in inducing hepatoma HepG2 cell apoptosis.Method] The cells were cultured in PRMI1640 medium and treated with experimental or clinically used paclitaxel in the different concentrations.The apoptosis in the cells was detected with the light microscope and the flow cytometry.Result] With the treatment of the clinical used paclitaxel in the final concentration of 0.5,1.0,2.0 mg/ml,the apoptosis can be induced very significantly in 24 hours,the cell apoptosis induced by 48 hours more intense.With experimental paclitaxel for 24 hours at a final concentration of 2.5,5.0,10.0 mg/ml,the apoptosis can be induced very significantly in 24 hours ; 2.5 mg/ml,apoptosis induced more intense by 48 hours.With the experimental paclitaxel concentration is 5.0,10.0 mg/ml,the rate of cell apoptosis gradually becomes low,the cells may lead to death.Conclusion] The concentration of experimental paclitaxel is higher than the clinically used paclitaxel in inducing hepatoma cell apoptosis.