遵义市规模养猪场大肠杆菌的耐药性检测

[目的]掌握遵义市规模养猪场大肠杆菌的耐药情况,为贵州省动物源性细菌监测提供数据。[方法]采集遵义市规模养猪场猪肛门拭子和总排污池新鲜粪便(或污水棉拭子)样品335份,分离出阳性大肠杆菌。采用微量肉汤稀释法对分离的大肠杆菌进行耐药性检测。[结果]分离并鉴定到334株阳性大肠杆菌,分离的大肠杆菌对7种抗菌药物的耐药率依次为:磺胺间甲氧嘧啶钠土霉素恩诺沙星头孢噻呋氟苯尼考环丙沙星庆大霉素,多重耐药性主要集中在4耐、5耐、6耐和7耐。[结论]遵义市规模养猪场大肠杆菌的耐药性严重及多重耐药性正在恶化,且猪场总排污池大肠杆菌对各药物的耐药性皆高于猪肛门拭子。

Drug Sensitivity Detection of Escherichia coli in Large-scale Pig Farms in Zhunyi City

[Objective] The research aimed to master the drug sensitivity of Escherichia coli in large-scale farms in Zhunyi City and provide data for monitoring animal-derived bacteria in Guizhou Province.[Method] 335 samples of pig anal swab and fresh manure from total sewage pool were collected large-scale pig farms in Zhunyi City to isolate positive E.coli.The drug sensitivity of the isolated E.coli was detected by using broth microdilution method.[Result] 334 strains of positive E.coli were identified.The drug sensitivity rates of the isolated E.coli to 7 kinds of antibacterial drugs were as follows：sulfamonomethoxine Sodium ＞ terramycin ＞ eurofloxacin ＞ ceftiofur ＞ florfenicol ＞ ciprofloxacin ＞ gentamicin.Multiple drug resistance was mainly concentrated in 4、5、6 and 7 resistant.[Conclusion] The drug sensitivity of E.coli was serious in large-scale pig farms in Zhunyi City and the multiple drug sensitivity was worse.The drug sensitivity of E.coli from total sewage pool was higher than that of swine anal swab.