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| 萼脊兰胚培养与快速繁殖研究 | |
| 引用本文： | 崔波,沈俊辉,马杰,顾东亚,蒋素华,叶永忠. 萼脊兰胚培养与快速繁殖研究[J]. 安徽农业科学, 2009, 37(13): 5861-5863 |
| 作者姓名： | 崔波 沈俊辉 马杰 顾东亚 蒋素华 叶永忠 |
| 作者单位： | 河南农业大学生命科学学院,河南郑州,450002;郑州师范高等专科学校生物技术研究所,河南郑州,450044;河南农业大学生命科学学院,河南郑州,450002;郑州师范高等专科学校生物技术研究所,河南郑州,450044 |
| 基金项目： | 河南省科技攻关项目(0524050005) |
| 摘 要： | [目的]研究萼脊兰的组织培养和快速繁殖技术。[方法]以萼脊兰的胚为材料，进行无菌胚培养，筛选适宜的培养基和适宜浓度的添加物。[结果]萼脊兰胚的萌发以1／4MS最适宜，培养80d时萌发率高达70％以上，其次为I／8MS、1／2MS。不同激素种类和不同浓度激素对胚萌发的影响比较大，以浓度0．5mg／LKT的效果最佳。有机添加物对萼脊兰胚的萌发有明显的影响，效果以10％椰子水最好。培养基中添加活性炭，对胚的萌发具有显著的促进作用。[结论]该研究建立起一套杂交兰快速繁殖及成苗的途径，可为以后的生产研究奠定理论基础。 |
| 关 键 词： | 萼脊兰 胚培养 原球茎 组织培养 快速繁殖 |
Embryo Culture and Rapid Propagation of Sedirea japonica |
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| CUI Bo et al. Embryo Culture and Rapid Propagation of Sedirea japonica[J]. Journal of Anhui Agricultural Sciences, 2009, 37(13): 5861-5863 | |
| Authors: | CUI Bo et al |
| Affiliation: | CUI Bo et al (Life Science College,Henan Agriculture University,Zhengzhou,Henan 450002) |
| Abstract: | [Objective]The research aimed to study the tissue culture and rapid propagation of Sedirea japonica.[Method]The embryos of S.japonica were taken as materials for culture to screen suitable medium and proper concentrations amendments.[Result]The best medium for the germination of the embryos of S.japonica was 1/4 MS,the germination rate after cultured for 80 d reached 70%,followed with 1/8 MS and 1/2 MS.Different hormone and different concentrations hormone had great effects on the germination of S.japonica ... |
| Keywords: | Sedirea japonica Embryo culture Protocorm like-body Tissue culture Rapid propagation |
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