| 牛LAP3基因内含子12和外显子13单核苷酸多态性 |
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| 引用本文： | 郑雪,鞠志花,李荣岭,李秋玲,张爱武,仲跻峰,王长法. 牛LAP3基因内含子12和外显子13单核苷酸多态性[J]. 中国农业科技导报, 2010, 12(5): 74-79. DOI: 10.3969/j.issn.1008-0864.2010.05.13 |
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| 作者姓名： | 郑雪 鞠志花 李荣岭 李秋玲 张爱武 仲跻峰 王长法 |
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| 作者单位： | (1.吉林农业大学中药材学院, 长春 130118,2.山东省农业科学院奶牛研究中心, 济南 250100) |
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| 基金项目： | 国家863计划项目，山东省良种工程项目，山东省攻关项目，山东省农业科学院高技术自主创新基金项目 |
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| 摘 要： | 采用单链构象多态性(single-strand conformation polymorphism,SSCP)、创造酶切位点PCR(created restriction site-PCR, CRS-PCR)、限制性片段长度多态性(restriction fragment length polymorphism, RFLP)以及直接测序方法,研究中国荷斯坦牛、鲁西黄牛和渤海黑牛LAP3基因第12内含子和第13外显子的单核苷酸多态性(SNP)。共检测到5个SNP,分别为24 794（T/G）、24 803(T/C)、24 846（T/C）、24 564（G/A）和25 415（T/C）,其中24 564（G/A）为首次报道的位点。经PCR-SSCP结合测序图谱发现24 794（T/G）、24 803(T/C)、24 846（T/C）位点完全连锁,但该连锁位点在鲁西黄牛群体中未检测到。5个SNP位点在中国荷斯坦牛、鲁西黄牛和渤海黑牛群体的优势等位基因相同,分别是T、T、T、G、T,其等位基因频率分别是T（CH 0.579/LY 1/BB 0.722）、T（CH 0.579/LY 1/BB 0.722）、T（CH 0.579/LY 1/BB 0.722）、G(CH 0.584/ LY 0.775/BB 0.500)和T(CH 0.596/LY 0.796/BB 0.750),多态信息含量均在0.25至0.5之间,属于中度多态。
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| 关 键 词： | 牛 LAP3基因 单核苷酸多态性 单链构象多态性 优势等位基因, |
| 收稿时间： | 2010-04-21 |
| 修稿时间： | 2010-06-03 |
Single Nucleotide Polymorphisms (SNPs) within intron 12 and exon 13 of LAP3 gene in Bos Taurus |
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| ZHENG Xue,JU Zhi-hua,LI Rong-ling,LI Qiu-ling,ZHANG Ai-wu,ZHONG Ji-feng,WANG Chang-fa. Single Nucleotide Polymorphisms (SNPs) within intron 12 and exon 13 of LAP3 gene in Bos Taurus[J]. Journal of Agricultural Science and Technology, 2010, 12(5): 74-79. DOI: 10.3969/j.issn.1008-0864.2010.05.13 |
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| Authors: | ZHENG Xue JU Zhi-hua LI Rong-ling LI Qiu-ling ZHANG Ai-wu ZHONG Ji-feng WANG Chang-fa |
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| Affiliation: | (1.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118|2.Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China) |
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| Abstract: | SNPs of the intron 12 and exon 13 of LAP3 were analyzed by PCR-SSCP, CRS-PCR, PCR-RFLP and sequencing in Chinese Holstein cattles(CH), Luxi Yellow cattles(LY) and Bohai Black cattles(BB). Five SNPs were detected, 24 794（T/G), 24 803(T/C), 24 846（T/C）, 24 564（G/A）and 25 415（T/C）, among them SNP 24 564（G/A） was new. Three SNPs of 24 794（T/G）, 24 803(T/C), 24 846（T/C）were linked completely and detected by PCR-SSCP, but they were not found in Luxi Yellow cattles. The alleles T, T, T, G and T were the dominant alleles in 3 breeds, and the allelic frequencies are T（CH0.579/LY 1/BB 0.722）, T（CH 0.579/LY 1/BB 0.722）, T（CH 0.579/LY 1/BB 0722）, G(CH 0.584/ LY 0.775/BB 0.500) and T (CH 0.596/LY 0.796/BB 0.750). The PIC of the 5 SNPs in these 3 breeds all belong to the midrange polymorphism (0.25
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| Keywords: | Bos Taurus LAP3 gene single nucleotide polymorphisms (SNPs) dominant allele |
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