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Inter simple sequence repeat (ISSR) markers as a tool for the assessment of both genetic diversity and gene pool origin in common bean (Phaseolus vulgaris L.)
Authors:M.Z. Galván  B. Bornet  P.A. Balatti  M. Branchard
Affiliation:(1) Instituto de Fisiología Vegetal, Facultad de Ciencias Agrarias y Forestales Universidad Nacional de La Plata, CC327, 1900 La Plata, Argentina;(2) Laboratoire de Biotechnologie et Physiologie Végétales, ISAMOR-Université de Bretagne Occidentale, 29280 Plouzané –, France;(3) Present address: IFREMER, Laboratoire DEL/MP/PN, BP21105, 44311 Nantes –, France
Abstract:
In this study, we report the use of ISSR to assess genetic diversity and to determine the relationships among ten cultivars of common bean developed in Argentina and three materials from France. ISSR markers resolved two major groups corresponding to the Andean and Mesoamerican gene pools of common bean. We compared the results of previous analysis, performed with RAPD markers (Galván et al., 2001), with the results generated by means of ISSR. It appears that ISSR are better tools than RAPDs to identify beans by gene pool of origin though they did not revealed as many differences between individuals as RAPDs. This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:common bean  fingerprints  ISSR  genetic relationships   Phaseolus vulgaris
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