利用悬浮培养和培养基选择改善火炬松的体细胞胚胎发生和植株再生(英文)

三个基固型的火炬松成熟合子胚被培养在附加 8mg·L-12 ,4 D ,4mg·L-1BA ,4mg·L-1KT ,5 0 0mg·L-1水解酪蛋白和 5 0 0mg·L-1谷氨酰胺的愈伤组织诱导培养基上诱导愈伤组织 .来自于子叶、胚轴和胚根的愈伤组织在附加 1 6mg·L-12 ,4 D ,0 8mg·L-1BA和 0 8mg·L-1KT的愈伤组织增殖培养基上培养 9周后 ,可获得 16 9%的胚性愈伤组织 .通过建立胚性细胞悬浮系和研究ABA、PEG和活性炭对体细胞胚成熟的促进作用 ,优化的体细胞胚胎发生体系被建立 .71棵再生小苗被用于移栽试验 ,2 3棵小苗在田间移栽成活

Enhanced Somatic Embryogenesis and Plant Regeneration from Embryogenic Cultures Derived from Mature Loblolly Pine Zygotic Embryos by Suspension Culture and Medium Selection

Mature zygotic embryos of three families of loblolly pine ( Pinus taeda L. ) were cultured on callus induction medium containing 8 mg L- 1 2,4-dichlorophenoxyacetic acid (2,4-D), 4 mg@ L- 1 6-benzyladenine (BA), 4 mg@ L- 1 kinetin (KT), 500 mg@ L- 1 casein hydrolysate, and 500 mg@ L- 1 glutamine for 9 weeks, callus was formed on cotyledons, hypocotyls, and radicles of mature zygotic embryos. Callus was sub-cultured on the callus proliferation medium with 1.6 mg@ L- 1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.8 mg@ L- 1 6-benzylade nine (BA), 0.8 mg@ L- 1 kinetin (KT) for 9 weeks. White translucent, glossy, mucilaginous embryogenic callus containing embryogenic suspensor masses (ESM) and immature somatic embryos was obtained, and the highest frequency of explants forming embryogenic callus was 16.9%. Embryogenic suspension cultures were established by culturing embryogenic callus in liquid callus proliferation medium. Liquid cultures containing embryogenic sus pension masses and immature somatic embryos were transferred to medium containing abscisic acid (ABA), poly ethylene glycols (PEG), or activated charcoal for enhancing the production of cotyledonary somatic embryos. After mature somatic embryos were cultured on medium containing indole-butyric acid (IBA), gibberellic acid (GA3),BA, and activated charcoal and being lowered sucrose concentration for 4 ～ 12 weeks, somatic embryos germinated to form regenerated plantlets. Seventy-one regenerated plantlets were transferred to a perlits:peatmoss:vermiculate ( 1: 1: 1 ) soil mixture, and 23 plantlets survived in the field.