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| 猪Ⅱ型圆环病毒Cap基因重组腺病毒表达载体的构建 | |
| 引用本文： | 马友记,柳纪省,赵兴绪,李志勇,殷相平,李宝玉.猪Ⅱ型圆环病毒Cap基因重组腺病毒表达载体的构建[J].甘肃农业大学学报,2005,40(6):713-717. |
| 作者姓名： | 马友记 柳纪省 赵兴绪 李志勇 殷相平 李宝玉 |
| 作者单位： | 1. 中国农业科学院兰州兽医研究所,甘肃,兰州,730046;甘肃农业大学动物医学院,甘肃,兰州,730070 2. 中国农业科学院兰州兽医研究所,甘肃,兰州,730046 3. 甘肃农业大学动物医学院,甘肃,兰州,730070 |
| 基金项目： | 重大基础研究“973”前期专项（2004CCA00500）. |
| 摘 要： | 通过细菌内同源重组的方法成功构建了含有猪Ⅱ型圆环病毒(PCV2)衣壳蛋白Cap基因的重组腺病毒表达载体。首先将Cap基因亚克隆连接到腺病毒穿梭载体pAdTrack-CMV上,再将重组质粒pAdTrack-CMV／Cap用Pme Ⅰ线性化后电转化携带有腺病毒骨架载体pAdeasy-1的大肠杆菌BJ5183感受态细胞,经细菌内同源重组产生重组腺病毒质粒pAdEasy-Cap。为进一步研究PCV2腺病毒活载体疫苗奠定了基础。 |
| 关 键 词： | 猪Ⅱ型圆环病毒 Cap基因 重组腺病毒 同源重组 |
| 文章编号： | 1003-4315（2005）06-0713-05 |
| 收稿时间： | 2005-08-26 |
| 修稿时间： | 2005-08-26 |
Construction and identification of recombinant adenovirus containing the cap gene of type Ⅱporcine circovirus |
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| MA You-ji,LIU Ji-xing,ZHAO Xing-xu,LI Zhi-yong,YIN Xiang-ping,LI Bao-yu.Construction and identification of recombinant adenovirus containing the cap gene of type Ⅱporcine circovirus[J].Journal of Gansu Agricultural University,2005,40(6):713-717. | |
| Authors: | MA You-ji LIU Ji-xing ZHAO Xing-xu LI Zhi-yong YIN Xiang-ping LI Bao-yu |
| Institution: | 1. Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Gansu, Lanzhou 730046; 2. Faculty of Veterinary Medicine, Gansu Agricultural University, Gansu, Lanzhou 730070, China |
| Abstract: | Recombinant replication-defective human adenovirus serotype 5 vector containing the Cap gene of type Ⅱ porcine circovirus (PCV2) was constructed using the method of homologous recombination in bacteria. Cap gene was subcloned into adenovirus shuttle vectors pAdTrack-CMV. The positive recom binant plasmids pAdTrack-CMV/Cap were linearized by PmeⅠ and electroporated into Ad-BJ5183 competent cells that had already been transformed with adenovirus skeletal vectors pAdeasy-1, the recombinant adenovirus plasmids pAdEasy-Cap were successfully obtained. These results indicated that recombinant adenovirus could be used on animals for PCV2 recombinant adenovirus vaccine. |
| Keywords: | PCV-2 Cap gene Recombinant adenovirus Homologous recombination |
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