猪源耐黏菌素大肠杆菌PFGE分型及耐药性研究

为了解河南省焦作市、许昌市规模化养殖厂耐黏菌素大肠杆菌的流行情况,对分离到的32株耐黏菌素大肠杆菌使用微量肉汤稀释法检测其药物敏感性,并采用脉冲场凝胶电泳(Pulsed field gel electrophoresis,PFGE)对分离株进行分型研究。结果显示,32株耐黏菌素大肠杆菌对四环素、多西环素、磺胺异噁唑、复方新诺明的耐药率均高达90%以上,32株猪源大肠杆菌可分为30种带型。结果表明,两地市养殖场猪源大肠杆菌耐药性普遍存在,且以多重耐药为主,PFGE分型结果显示,32株耐黏菌素大肠杆菌整体上表现出较大的遗传多样性,但不同菌株存在着不同程度的亲缘关系。

Study on PFGE Typing and Drug Resistance of Swine Escherichia coli

To investigate the epidemic status of the Escherichia coli isolated from swine, thirty-two E.coli isolates collected from Jiaozuo, Xuchang, Kaifeng city, Henan province were subject to identifications of the antibiotics resistance and PFGE typing by microbroth dilution method and Pulsed field gelelectrophoresis. Result showed that 32 clinic isolates were resistant to tetracycline, Doxycycline, Sulfonamidoxazole, Paediatric Compound Sulfamethoxazole Tablets, drug resistance rates were all above 90%. 32 swine E.coli isolates can be divided into 30 PFGE types. The study indicated that the resistance of E.coli in the farms was widespread, and multidrug resistance accounted for the most. PFGE results showed that the prevalence of E.coli in the farms of Jiaozuo, Xuchang, Kaifeng may be caused by the spread of many kinds of genotypes of stains.