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| 稻瘟菌MoHYR1蛋白的原核表达与纯化 | |
| 引用本文： | 钱恒伟,迟梦宇,赵颖,梁文星,黄金光,李宝笃.稻瘟菌MoHYR1蛋白的原核表达与纯化[J].植物病理学报,2018,48(1):141-144. |
| 作者姓名： | 钱恒伟 迟梦宇 赵颖 梁文星 黄金光 李宝笃 |
| 作者单位： | 青岛农业大学植物医学学院, 青岛 266109 山东省植物病虫害综合防控重点实验室, 青岛 266109 |
| 基金项目： | 国家自然基金(31471735); 山东省“泰山学者”建设工程专项经费(6631115038) |
| 摘 要： | 正稻瘟菌(Magnaporthe oryzae)是高等丝状子囊真菌,由其侵染水稻引发的稻瘟病是水稻第一大病害,生产上造成了巨大的经济损失。在植物与病原菌的互作过程中,植物会产生防御应答反应,其中包括活性氧自由基(reactive oxygen species,ROS)。ROS在植物抵御病原菌的侵入以及病原 |
| 收稿时间： | 2017-03-01 |
Prokaryotic expression and purification of MoHYR1 protein from Magnaporthe oryzae |
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| QIAN Heng-wei,CHI Meng-yu,ZHAO Ying,LIANG Wen-xing,HUANG Jin-guang,LI Bao-du.Prokaryotic expression and purification of MoHYR1 protein from Magnaporthe oryzae [J].Acta Phytopathologica Sinica,2018,48(1):141-144. | |
| Authors: | QIAN Heng-wei CHI Meng-yu ZHAO Ying LIANG Wen-xing HUANG Jin-guang LI Bao-du |
| Institution: | College of Plant Health and Medicine, Qingdao Agriculture University, Qingdao 266109, China Key Lab of Integrated Crop Disease and Pest Management of Shandong province, Qingdao 266109, China |
| Abstract: | The gene MoHYR1 is important for full virulence and H2O2 tolerance in the rice blast fungus Magnaporthe oryzae. MoHYR1 was obtained by PCR with the template of M.oryzae cDNA and cloned into a prokaryotic expression vector pHAT2. The protein MoHYR1 was successfully expressed in strain Escherichia coli BL21(DE3) with an estimated molecular mass of 20 kDa. The protein was then purified by affinity chromatography and gel filtration. Finally, high purity of MoHYR1 was obtained, which contributes to further study on its structure and function. |
| Keywords: | Magnaporthe oryzae MoHYR1 prokaryotic expression affinity chromatography gel filtration |
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