| 影响文心兰原球茎增殖生长诸因素的研究 |
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| 引用本文： | 杨金凤,李旭,廉美兰,朴炫春.影响文心兰原球茎增殖生长诸因素的研究[J].北方园艺,2009(3). |
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| 作者姓名： | 杨金凤 李旭 廉美兰 朴炫春 |
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| 作者单位： | 延边大学,长白山生物功能因子省部共建教育部重点实验室,吉林,延吉,133002;延边大学,长白山生物功能因子省部共建教育部重点实验室,吉林,延吉,133002;延边大学,长白山生物功能因子省部共建教育部重点实验室,吉林,延吉,133002;延边大学,长白山生物功能因子省部共建教育部重点实验室,吉林,延吉,133002 |
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| 摘 要： | 以文心兰茎尖为外植体,诱导原球茎,并对影响原球茎增殖的几种因素进行了研究.结果表明:文心兰茎尖在1/2 MS+BA 2 mg/L+NAA 0.3 mg/L培养基上,离体培养大约30 d后产生原球茎;原球茎在1/2 MS+BA 1 mg/L+NAA 0.2 mg/L培养基上增殖速度最快,增殖系数可达2.6;与果糖和葡萄糖相比,蔗糖更有利于原球茎增殖;30 g/L的蔗糖对原球茎增殖生长有促进作用.
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| 关 键 词： | 文心兰 原球茎 组织培养 |
Study on Several Factors Affecting Protocorm-Like Body of Oncidium in vitro |
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| YANG Jin-feng,LI Xu,LIAN Mei-lan,PIAO Xuan-chun.Study on Several Factors Affecting Protocorm-Like Body of Oncidium in vitro[J].Northern Horticulture,2009(3). |
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| Authors: | YANG Jin-feng LI Xu LIAN Mei-lan PIAO Xuan-chun |
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| Institution: | Key Laboratory of Organism Functional Factors of the Changbai Mountain;Ministry of Education;Yanbian University;Yanji;Jilin 133002;China |
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| Abstract: | In order to establish propagation system of protocorm-like body(PLB) in Oncidium during tissue culture,the meristem was used as explants for inducing PLB,the result showed that after cultured in vitro for one month,the shoot of Oncidium Luridum cvs could generate PLBs on culture medium involving 1/2MS,BA(2 mg/L) and NAA(0.3 mg/L).PLBs had the fast proliferation speed on culture medium involving 1/2MS,BA(1 mg/L) and NAA(0.3 mg/L),K-factor could reach to 2.6.Compared to fructose and glucose,sucrose was best f... |
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| Keywords: | Oncidium Protocorm-like body Tissue culture |
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