Effective Diploidization of Microspore-Derived Haploids of Rape (Brassica napus L.) by In Vitro Colchicine Treatment |
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| Authors: | Regine Mathias G Röbbelen |
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| Institution: | Dr. Regine;Mathias, Prof. Dr. G. Röbbelen, Institut für Pflanzenbau und Pflanzenzüchtung, Von-Siebold-Straße 8, W-3400 Göttingen (Germany). |
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| Abstract: | A technique for the in vitro colchicine treatment of microspore-derived haploids of rape (Brassica napus L.) is presented. This allows effective and time-saving chromosome doubling. The most effective method consists of an application of 50 mg/1 colchicine in a liquid B5 medium to the rooted micro-spore-derived plantlets for 4–8 days. By this treatment more than 50 % of the plants developed at least diploid sectors. A change of medium after some days may even increase this diploidization rate. |
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| Keywords: | Brassica napus microspore culture haploids colchicine treatment diploidization |
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