| 单叶刺槐组织培养研究 |
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| 引用本文： | 朱建峰,杨敏生,王进茂.单叶刺槐组织培养研究[J].北方园艺,2010(2):175-178. |
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| 作者姓名： | 朱建峰 杨敏生 王进茂 |
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| 作者单位： | 河北农业大学,林学院,河北,保定071001;河北农业大学,林学院,河北,保定071001;河北农业大学,林学院,河北,保定071001 |
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| 基金项目： | 科技部“十一五”科技支撑计划资助项目(2006BAD03A0106);;国家林业局科技推广资助项目(200603) |
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| 摘 要： | 以单叶刺槐(Robinia. pseudoacaciaf. unifolia)带腋芽的茎段为外植体,探讨不同植物激素6-BA、IBA、NAA对试管苗增殖以及植株再生的影响,建立单叶刺槐离体培养技术体系。结果表明:启动最适培养基为MS+6-BA1.0mg/L;增殖最适培养基为MS+6-BA1.0mg/L+NAA0.2mg/L;生根培养基为1/2MS+IBA50mg/L,预培养7d,再转至1/2MS0培养10d;在此体系中诱导率可达95.0%,增殖系数可达5.72,生根率83.3%,移栽成活率可达到100%。
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| 关 键 词： | 单叶刺槐 组织培养 快速繁殖 |
Tissue Culture of Robinia. Pseudoacaciaf. Unifolia |
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| ZHU Jian-feng,YANG Min-sheng,WANG Jin-mao.Tissue Culture of Robinia. Pseudoacaciaf. Unifolia[J].Northern Horticulture,2010(2):175-178. |
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| Authors: | ZHU Jian-feng YANG Min-sheng WANG Jin-mao |
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| Institution: | Forestry College;Agricultural University of Hebei;Baoding;Hebei 071001 |
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| Abstract: | Stems with axillaries bud of Robinia.pseudoacaciaf.unifolia were used as explants,this paper probed into the effects of 6-BA,NAA,IBA on proliferation and plantlet regeneration of Robinia.pseudoacaciaf.unifolia.The results showed that the medium suitable for bud germination was MS+6-BA 1.0 mg/L;The optimum multiplication culture medium was MS+BA 1.0 mg/L+NAA 0.2 mg/L;The rooting culture medium was that the clones were cultured 7 d in 1/2MS+IBA 50 mg/L,then were cultured 10 d in 1/2MS0 medium.In this system,t... |
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| Keywords: | Robinia pseudoacaciaf unifolia tissue culture rapid propagation |
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