| 山东茶树良种组织培养及繁殖能力的研究 |
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| 引用本文： | 孙仲序,刘静,王玉军,刘杰,杨德平.山东茶树良种组织培养及繁殖能力的研究[J].茶叶科学,2000,20(2):129-132. |
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| 作者姓名： | 孙仲序 刘静 王玉军 刘杰 杨德平 |
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| 作者单位： | 1. 山东农业大学,山东泰安,271018
2. 泰安市林科所,山东泰安,271000 |
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| 摘 要： | 取茶树嫩茎段作外植体进行离体培养 ,以MS作基本培养基 ,经添加不同激素水平及组合的试验 ,得到最佳分化率 83% ,其培养基配方为MS +BA 1 5+ZT 0 5;最佳生根率 80 % ,其培养基配方为 1/ 2MS +IBA0 2 +2 0g糖。选出了最佳移栽基质为蛭石 +壤土 +木屑 ,其配比为 2∶1∶1,移栽成活率达 85%。一个外植体经三次继代培养可繁殖 4 7棵苗。
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| 关 键 词： | 茶树 组织培养 繁殖 |
Studies on Tissue Culture and Fertility of the Improved Tea Cultivars in Shandong Province |
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| SUN Zhong-xu,WANG Yu-jun,LIU Jie,LIU Jing,YANG De-ping.Studies on Tissue Culture and Fertility of the Improved Tea Cultivars in Shandong Province[J].Journal of Tea Science,2000,20(2):129-132. |
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| Authors: | SUN Zhong-xu WANG Yu-jun LIU Jie LIU Jing YANG De-ping |
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| Institution: | SUN Zhong xu 1,LIU Jing 2,WANG Yu jun 1,LIU Jie 1,YANG De ping 2 [WT6BX] |
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| Abstract: | The best media and hormone combinations for differentiation and rooting were found out via tissue culture with tea shoot segments as explants and MS as basic medium added with different levels of hormones The highest differentiation ratio (83%) was obtained on the MS medium added with 1 5 mg/L BA +0 5mg/L ZT and the highest root ratio (80%) was obtained on the MS medium added with 0 2mg/L IBA+20g saccharide The rooted shoots were then transplanted to different media, and the highest survival ratio (85%) was achieved with a medium composed of vermiculite+loam+woodbits (2:1:1) An explant could reproduce 47 plantlets after three subcultures |
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| Keywords: | Tea plant Tissue culture Propagation |
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