高氟对雏鸡肝细胞周期和凋亡影响的研究

选用300只1日龄Avian肉鸡健雏,随机分为4组,分别饲以对照日粮(F 23 mg·kg~(-1))和高氟口粮(F 400 mg·kg~(-1)>,Ⅰ组;F 800 mg·kg~(-1),Ⅱ组;F 1 200 mg·kg~(-1),Ⅲ组)42 d,第14、28、42天每组随机抽取5只,利用流式细胞术研究高氟对雏鸡肝细胞周期和凋亡的影响.结果显示:高氟Ⅱ组和Ⅲ组肝脏质最显著低于对照组(P<0.05),脏器指数显著高于对照组(P<0.05).流式细胞仪测定,14 日龄时高氟Ⅱ组和高氟Ⅲ组肝细胞G_0/G_1期极显著升高(P<0.01),S期、G_2+M期和增殖指数(PI)与对照组相比不同程度地降低;28和42日龄时高氟Ⅱ组和高氟Ⅲ组肝细胞G_0/G_1极显著降低(P<0.01),S期和PI值显著或极显著升高(P<0.05或P<0.01),G_2+M期无显著变化.14、28和42日龄时,高氟Ⅱ和Ⅲ组肝细胞同时凋亡率均极显著高于对照组(P<0.01).日粮含氟800及1 200 mg·kg~(-1)时可引起肝细胞增殖能力增强,凋亡细胞显著增多.

Effect of Dietary High Fluorine on the Cell Cycle and Apoptosis of Liver in Chickens

300 one-day-old Avian broilers were divided into four groups and fed on Control diet(F 23 mg·kg~(-1)) and High fluorine diets(F 400 mg·kg~(-1), High fluorine group Ⅰ; F 800 mg·kg~(-1), High fluorine group Ⅱ; F 1 200 mg·kg~(-1), High fluorine group Ⅲ) for 6 weeks, the effect of di-etary high fluorine on the cell cycle and apoptosis of liver in chickens were researched by the methods of flow cytometry (FCM) at 14,28,42 days old. The weight of liver was significantly decreased and the relative weight of liver was significantly increased in high fluorine group Ⅱ and Ⅲ when compared with control group. By FCM, the G_0/G_1 phase was significantly increased, and S phase, G_2+M phase and PI(Proliferating index) decreased in diversity in high fluorine groups Ⅱ and Ⅲ at 14 days of age. The G_0/G_1 phase was significantly decreased, S phase and PI significantly increased, and G_2+M phase was not changed in high fluorine group Ⅱ and Ⅲ when compared with those of control group at 28 and 42 days of age. The apoptotic rate was higher in high fluorine group Ⅱ and Ⅲ than that in control group at 14, 28 and 42 days of age. The result showed that fluorine at 800 and 1 200 mg·kg~(-1) in diet could cause enhanced multiplication capaci-ty of hepatoeyte and increased apoptotic.